1 / 14

Biochemistry I

Biochemistry I. Labs 22,23,24. Exoenzymes – Lab 22. Bacteria make enzymes to digest macromolecules outside of the cell The smaller sub units of the enzymatic reaction are then able to be transported across the cell membrane This capacity is a characteristic of many Gram Positive bacteria.

livana
Download Presentation

Biochemistry I

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript

  1. Biochemistry I Labs 22,23,24

  2. Exoenzymes – Lab 22 • Bacteria make enzymes to digest macromolecules outside of the cell • The smaller sub units of the enzymatic reaction are then able to be transported across the cell membrane • This capacity is a characteristic of many Gram Positive bacteria

  3. Hydrolysis of starch • Starch is composed of glucose molecules connected by glycosidic linkages • When the glycosidic linkages are broken, individual sugar molecules are produced • Linkages are broken in the presence of Water and the enzyme amylase

  4. Hydrolysis of Gelatin • When water and an enzyme are added to a protein, the peptide bond in proteins are broken, producing individual amino acids

  5. Gelatin Hydrolysis in the tube

  6. Carbohydrates and Sugars

  7. Glycolysis

  8. Sugar fermentation- Carbohydrate metabolism

  9. Fermentation • Phenol red – pH indicator is red in a neutral environment and yellow is the result of an acidic pH • Gas – Gas is a by -product of the fermentation reaction -

  10. www.jlindquist.net/generalmicro/dfnewgfbpage.html

  11. Triple Sugar Iron • a. metabolize lactose and/or sucroseb. conduct fermentation to produce acidc. produce gas during fermentationd. generate H2S.

  12. TSI Results

  13. Results

More Related