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Regulation of Tracheal Fusion by Arf like GTPase Confusion

Regulation of Tracheal Fusion by Arf like GTPase Confusion. DB. DT. LT. GB. Arf like GTPase. Arf family GTPase ( ADP-ribosylation factor GTP binding proteins): Arf, Arf like and Sar Arf like GTPase Arl1, ARFRP : TGN ( trans -Golgi network) Arl2, Arl3: Microtubule biogenesis

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Regulation of Tracheal Fusion by Arf like GTPase Confusion

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  1. Regulation of Tracheal Fusion by Arf like GTPase Confusion DB DT LT GB

  2. Arf like GTPase • Arf family GTPase ( ADP-ribosylation factor GTP binding proteins): Arf, Arf like and Sar • Arf like GTPase • Arl1, ARFRP : TGN (trans-Golgi network) • Arl2, Arl3: Microtubule biogenesis • Cfu is Arl3 homologue

  3. Confusion Expression in Tracheal Fusion Cells Merge Cfu Dys

  4. Cfu Mutants Show Tracheal Fusion Defects control Cfu RNAi w1118 d10234/d10234

  5. Cfu Mutants d10234 d10234/d10234 w1118

  6. Fusion Cell Identity D10234/TM3 twi-GFP D10234/D10234 Dys DSRF

  7. Fusion Process E-Cadherin Cytoskeletal track: shortstop, F-actin, microtubule Peter A. Kolodziej et. al Development, 2003

  8. Cfu localization in Fusion Cells Cfu Merge Actin Tublin

  9. Sequence Analysis of Cfu G2A T31N: negative Q72L: active

  10. Cfu Q72L Transfected S2 Cell Cfu Merge Actin Tublin Dapi Cfu Q72l S2

  11. Potential Role of Cfu in Trafficing No Colchicine

  12. Potential Role of Cfu in Trafficing Colchicine: prevent microtubule polymerization

  13. Potential Role of Cfu in Trafficing No Latrunculin Latrunculin: prevent actin polymerization

  14. Potential Role of Cfu in Trafficing Latrunculin: prevent actin polymerization

  15. Future Directions • UAS-cfu, UAS-cfuQ72L, UAS-cfu T31N rescue cfu mutants • Endsout to generate cfu mutants • Time-lapse movie: UAS-actin-GFP / btl, gal4; d10234/d10234 • UAS-tubulin-GFP / btl, gal4; d10234/d10234 UAS-shot-GFP / btl, gal4; d10234/d10234 • RNAi screening to identify genes interact with Cfu

  16. Endsout wi I-SceI site I-SceI site * FRT FRT Flip, I-SceI * Target gene *

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