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Ex. 16 Oxygen Requirements

Ex. 16 Oxygen Requirements. Oxygen Requirements. 1. Aerobes 2. Anaerobes 3. Facultative 4. Microaerophilic. Agar Stabs & Agar Shakes. 1. Liquify 6 NA Deeps in boiling water 2. Boil for 10 minutes to drive out dissolved oxygen 3. Allow 3 NA Deeps to cool and solidify Agar Stabs

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Ex. 16 Oxygen Requirements

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  1. Ex. 16 Oxygen Requirements

  2. Oxygen Requirements • 1. Aerobes • 2. Anaerobes • 3. Facultative • 4. Microaerophilic

  3. Agar Stabs & Agar Shakes • 1. Liquify 6 NA Deeps in boiling water • 2. Boil for 10 minutes to drive out dissolved oxygen • 3. Allow 3 NA Deeps to cool and solidify • Agar Stabs • 4. Temper 3 NA Deeps in water bath at 55 degrees C • Agar Shakes

  4. Agar Stabs • 1. Single stab with Inoculating needle – straight down, straight back up • 1. Micrococcus luteus • 2. Staphylococcus epidermidis • 3. Clostridium sporogenes • Clostridium rubrum • Clostridium butrycum

  5. Agar Shakes • 1. Inoculate with Inoculating Loop - “shake” test tube so bacteria are equally distributed • 1. Micrococcus luteus • 2. Staphylococcus epidermidis • 3. Clostridium sporogenes • Clostridium rubrum • Clostridium butrycum

  6. Agar Stab • Aerobic

  7. Agar Stab • Anaerobic

  8. Agar Stab • Facultative

  9. Agar Stab • Microaerophilic

  10. Fluid Thioglycollate Broths (FTB) • Sodium thioglycollate • Binds to and removes free oxygen • Resazurin • Anaerobic indicator • O2 - PINK • No O2 - Clear

  11. Oxygen Reqirements

  12. Aerobic Microaerophilic Anaerobic

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