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Supplemental Figure 1.

Supplemental Figure 1. a. db/m db/db db/db + fasudil. b. db/m db/db db/db + fasudil. c. db/m db/db db/db + fasudil. NS. Supplemental Figure 2. Hypoxia. - + - +. Y-27632. - - + +. Supplemental Figure 3. a. b. c. DFX.

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Supplemental Figure 1.

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  1. Supplemental Figure 1. a db/mdb/dbdb/db + fasudil b db/mdb/dbdb/db + fasudil c db/m db/db db/db + fasudil NS

  2. Supplemental Figure 2. Hypoxia - + - + Y-27632 - - + +

  3. Supplemental Figure 3. a b c DFX DFX DFX DFX DFX DFX DFX - + - + - + - + - + - + - + - + - + - + - + - + - + - + Y-27632 Y-27632 Y-27632 Y-27632 Y-27632 Y-27632 Y-27632 - - + + - - + + - - + + - - + + - - + + - - + + - - + + d e f g h ** IB: HIF-1α IB: β-actin DFX - + - + - + Y-27632 - - + + - + MG132 - - - - + + ** ** ** ** ** ** ** ** ** ** ** ** ** **

  4. Supplemental Figure 4. Polyubiquitinated HIF-1α IP:HIF-1α IB:Ub IB:HIF-1α IB: β-actin Y-27632 - + MG132 + +

  5. Supplemental Figure 5. Rho-kinase activity IB: p-MYPT1 IB: MYPT1 DFX - +

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