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Axl-2

Luciferase siRNA. No 1st Ab. Axl siRNA. 800000. 700000. 600000. 500000. RLU. 400000. 300000. 200000. 100000. 0. not transfected. GL2. Axl-4. D-3. D-4. GL2. Fig.1. A. Axl-2. Axl-4. D-1. D-2. D-3. D-4. B. 300. Luc. ferase GL2. i. si. RNA. i. 250. f. or. w.

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Axl-2

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  1. Luciferase siRNA No 1st Ab Axl siRNA 800000 700000 600000 500000 RLU 400000 300000 200000 100000 0 not transfected GL2 Axl-4 D-3 D-4 GL2 Fig.1. A. Axl-2 Axl-4 D-1 D-2 D-3 D-4 B. 300 Luc ferase GL2 i si RNA i 250 f or w ard : CGU ACG CGG AAU ACU UCG ATT r everse: UCG AAG UAU UCC GCG UAC GTT 200 Dhar m ac o n - 1 MFI 150 for w ard : GAA AGA AGG AGA CCC GTT ATT r everse: TAA CGG GTC TCC TTC TTT CTT 100 Dhar m ac o n - 2 for w ard : CCA AGA AGA TCT ACA ATG GTT 50 reverse: CCA TTG TAG ATC TTC TTG GTT Dhar m ac o n - 3 0 GL2 Axl-2 Axl-4 D-1 D-2 D-3 D-4 no 1st Ab for w ard : GGA ACT GCA TGC TGA ATG ATT C. reverse: TCA TTC AGC ATG CAG TTC CTT Dhar m ac o n - 4 for w ard : GAA GGA GAC CCG TTA TGG ATT reverse: TCC ATA ACG GGT CTC CTT CTT A x l - 2 for w ard : GAC ATC CTC TTT CTC CTG CTT reverse: GCA GGA GAA AGA GGA TGT CTT A x l - 4 for w ard : GAT TTG GAG AC ACA CTG ATT reverse: TCA GTG TGT TCT CCA AAT CTT

  2. Fig.2. 10 Axl-4 Dkk3 Ang-2 1 GL2 Axl DKK3 Fold of change Ang-2 -10 -100 siRNA

  3. Axl -actin BaF3 BaF3/Axl D. 350000 300000 250000 RLU 200000 150000 100000 50000 0 sup 7C3 3C7 6A8 4F8 3B3 2C9 8B5 7B4 7D5 6G2 3G9 3G4 13F6 3C12 8F11 2B10 12A11 13H10 control AxlFc (200ng/ml) Fig.3. A. B. C. 120 250000 100 200000 80 RLU Viability(%) 150000 BaF3 60 BaF3 Axl 100000 40 50000 20 0 0 12.5 25 50 100 200 0 0 0.1 0.25 0.5 1.25 2.5 Gas6 (ng/ml) AxlFc (g/ml)

  4. Fig.4. A. human Axl.ECD.His mouse Axl.Fc B. 3 2.5 2 control OD450nm 4F8 8B5 1.5 12A11 3G9 1 0.5 0 huAxl.Fc huMer.fc huTyro.Fc Antigen concentration (10ug/ml)

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