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FSC

pDC. medium. Flu. CL097. 20 %. 85 %. 95 %. CD40. BDCA2. CD123. FSC. GEN2.2 cells. medium. Flu. CL097. 25 %. 82 %. 97 %. CD40. SSC. FSC. FSC. 100. GEN2.2. 2.5. GENshTLR7. GEN2.2. 80. GENshTLR7. 2.0. 60. % CD40 positive cells. 1.5. relative Ct. 40. 1.0. 20. 0.5.

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FSC

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  1. pDC medium Flu CL097 20 % 85 % 95 % CD40 BDCA2 CD123 FSC GEN2.2 cells medium Flu CL097 25 % 82 % 97 % CD40 SSC FSC FSC 100 GEN2.2 2.5 GENshTLR7 GEN2.2 80 GENshTLR7 2.0 60 % CD40 positive cells 1.5 relative Ct 40 1.0 20 0.5 0.0 0 TLR7 TLR9 medium Flu CL097 CpG A CpG B A B C D Figure 1

  2. 10000 IFN α IL-6 8000 IL-8 6000 TNFα pg/ml 4000 2000 0 medium Flu CL097 medium Flu CL097 0.2 % 56 % 7 % medium Flu CL097 IFN a BDCA-4 A B C D E Figure 2

  3. 10000 medium Flu 8000 CL097 (pg/ml) 6000 4000 IFN α 2000 0 0.5h 2h 4h 24h A B C D E F Figure 3

  4. well well + anti-IFNα/β 100 100 transwell well transwell + anti-IFNα/β transwell 80 80 60 60 % TRAIL positive cells % TRAIL positive cells 40 40 20 20 0 0 medium Flu CL097 IFN α medium Flu CL097 IFN α A B C D Figure 4

  5. medium Flu CL097 2h 2h 2h 2h 0.5h 0.5h 0.5h 0.5h p-STAT1 p-STAT2 IFN a actin a medium Flu CL097 IFN IFN a 5 % medium 30 % Flu 29 % CL097 65 % FSC phospho-STAT1 A B C D Figure 5

  6. IFN a medium Flu CL097 4 % 94 % 94 % 92 % medium 2 % 1 % 3 % 85 % LY a medium Flu CL097 IFN 7 % 10 % 10 % 90 % SB SSC TRAIL 8000 medium LY 6000 SB CXCL10 (pg/ml) 4000 2000 0 medium Flu CL097 IFN a A B C D E F Figure 6

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