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PRESS CONFERENCE. GENETIC MODIFIED FOOD. Mechanism of Genetically Modified Food--GM food (There are totally 2 methods) Applications of Genetic Modification Pros and Cons of GM food News Government’s action. Genetically Modified Food. Mechanism of GM Food(1).
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PRESS CONFERENCE GENETIC MODIFIED FOOD
Mechanism of Genetically Modified Food--GM food (There are totally 2 methods) Applications of Genetic Modification Pros and Cons of GM food News Government’s action Genetically Modified Food
Mechanism of GM Food(1) • 1)Isolating/synthesizing the target gene • 2)Inserting the target gene into a vector, i.e. an intermediate carrier • 3)Transferring the vector containing the target gene into a host cell and cloning the DNA • 5) Mixing plant cells with host cells
Mechanism1 : Isolating/synthesizing the target gene • The ‘shotgun’ approach, using type II restriction enzyme(restriction endonuclease ) - the enzyme cuts at recognition sites, to obtain a desired gene - sticky ends or blunt ends produced (there are figures later) • Making a copy of the gene from its mRNA - poly-T oligomerbinds to the complementary poly-A tail of the mRNA - a single strand of DNA copy formed - digesting mRNA - a double-stranded cDNA produced
Mechanism1 : Inserting the target gene into a vector • Step 1) Prepare a target gene with sticky end if it has blunt end - Short pieces of DNA are joined at the end of the gene - restriction enzymes cut linkers • Step 2) Prepare the plasmid DNA (a figure later) as vector - centrifugation • Step 3) Combine the target gene and the plasmid DNA by DNA ligase - To have the same sticky end,the same restriction enzyme is used to cut the vector
Plasmid DNA • Circular DNA(2), which is smaller and lighter than main chromosomal DNA(1)
Mechanism1 : Transferring the vector into a host cell (transformation) and Cloning the DNA • Step 1) Adding the plasmid vector into a flask containing culture of a host cell • Step 2) Generating temporary pores on the surface of the host cell • Step 3) Allowing the plasmid vector to enter the host cell • Step 4) Placing the host cell in a dish with nutrients
Mechanism1: Mixing plant cells with host cells • Using Agrobacterium tumefaciens or Agrobacterium rhizogenes as vectors • Introducing Ti-plasmid into the plant cells • The whole plant can be regenerated from a transformed leaf disc
Mechanism of GM Food(2) • Antisense approach • Switching off or turning down (underexpressing) a resident gene in the organism • Antisense RNA is produced
Applications of Genetic Modification in Food Production • GM Food -Tomatoes and tomato paste -Corn maize -GM soya beans • GM processing aid -A kind of GM organism making a product without GM material • GM ingredients in animal feed
Benefits of GM food • higher yields • increased muscle bulk (animals) • the ability to resist disease, pests or chemicals • a prolonged shelf-life • higher nutritional value • reduced allergens • the ability to grow in environments that are usually too hostile.
Disadvantages of GM food • Unintended modification of similar species due to cross pollination • Upsetting the ecosystem • Development of super pests • Problems about religious, health, etc • Destroying the normal farming system • Interfering the normal DNA system • Widening the financial gaps between developed and developing countries
SOURCES: New Scientist (issue 2530) Letter to the Editor, 17 December 2005 • allergenic potential of genetically modified foods (e.g. mouse strains in peas ) • Existing tests provide no direct immunological information and cannot rule out allergenic proteins • regulators should demand full sequencing of the transgenic proteins in plants
SOURCES: ISIS Press Release 16December,2005 • Genetically modified DNA from any part of a GM plant can enter the environment unobserved • causing a wave of “superweeds” • ‘co-existence’ is biologically impossible
no existing public records of contamination incidents or other problems associated with GM crops • strict rules with liability regulations should be applied by governments to stop the unauthorized spread of GM seeds and products • data used by the Government to approve GM foods should be accessed freely
SOURCES: BBC News 8 December 2005 • Modified animals: 27%(2003) 32%(2004) • Total experiments: just over 2.85 million • Number of animals used in research: 2.78 million (a rise of 2.1% on 2003 figures)
SOURCES: GM Free Cymru [Wales] Press Notice 25thNovember 2005 • Within three weeks of birth, - 55% of the rats fed on GM soya died - 9% of the rats died in the control group • mice fed on GM soya experienced a slowdown in cellular metabolism and modifications to liver and pancreas • introduction of genes from a bean variety into a GM pea led to the creation of a novel protein which caused inflammation of the lung tissue of mice.
What should HK government do? • Setting up mandatory labelling system for GM food • informing the public the pros and cons of GM foods • building up its own expertise with public health professionals and GM food-related scientists
What did China government do? • On March 20th 2002 , the Regulations on the Administration of the Safety of Transgenic Agricultural Products (RASTAP) became effective • According to RASTAP and its measures, two methods of application are required to control the safety of transgenic products in international trade.
Regulations on the Administration of the Safety of Transgenic Agricultural Products (RASTAP) • First, Chinese importers or foreign exporters must apply for the transgenic products which may be imported later to be risk assessed. • Second, foreign exporters must label their transgenic products.