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The comparing of natural antibacterial substance on E. Coli

The comparing of natural antibacterial substance on E. Coli. Manhasset Science Research Jean Cheng Period 4. Need. Figure 1: Antimicrobial drugs resistance pattern of urinary E. coli from Southern Brazil. http://www.scielo.br/img/revistas/bjm/v39n4/arq27_fig01.jpg. Knowledge Base.

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The comparing of natural antibacterial substance on E. Coli

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  1. The comparing of natural antibacterial substance on E. Coli Manhasset Science Research Jean Cheng Period 4

  2. Need Figure 1: Antimicrobial drugs resistance pattern of urinary E. coli from Southern Brazil http://www.scielo.br/img/revistas/bjm/v39n4/arq27_fig01.jpg

  3. Knowledge Base Figure 2: Chemical formula for Penicillin http://itech.dickinson.edu/chemistry/wp-content/uploads/2008/04/penicillin-core1.png

  4. Knowledge Base Figure 2: Chemical formula for Penicillin http://itech.dickinson.edu/chemistry/wp-content/uploads/2008/04/penicillin-core1.png

  5. Knowledge Base (cont) Figure3: Penicillin attacking the peptidocglycan http://upload.wikimedia.org/wikipedia/commons/thumb/8/8b/Gram_negative_cell_wall.svg/800px-Gram_negative_cell_wall.svg.png

  6. Knowledge Base (cont) Figure3: Penicillin attacking the peptidocglycan http://upload.wikimedia.org/wikipedia/commons/thumb/8/8b/Gram_negative_cell_wall.svg/800px-Gram_negative_cell_wall.svg.png

  7. Knowledge Base (cont) Figure 4: Tea tree’s terpene chemical formula http://www.wacker.com/cms/appL/mdm_images/R67_IV-1_-432011277_8959.jpg Figure 5: Pectin Chemical formula http://sci-toys.com/ingredients/pectin_2.gif

  8. Knowledge Base (cont) Figure 6: Aloe vera http://apps.who.int/medicinedocs/fr/d/Js2200e/5.html

  9. Knowledge Base (cont) Figure 7: Citral http://www.impgc.com/images/Structures/Citral.bmp

  10. Literature Review Figure 8:Chemical Change in Allicin subjected to different conditions. (Ariga, et al. 2008) http://media-2.web.britannica.com/eb-media/33/7433-004-96DFD197.gif

  11. Literature Review (cont) Figure 9: Antimicrobial activity of aloe vera gel (Alemdar, et al. 2009)

  12. Literature Review (cont) Figure 10:Yuzu fruit seed oil on different microbial (Nakazono, et al. 2006)

  13. Literature Review (cont) Figure 11:12 different essential oil on different species of bacteria. (Rusenova, et al. 2009)

  14. Purpose • Find which natural substance is best in inhibiting E. coli. • Null Hypothesis: No effect • Alternative Hypothesis: Tea tree oil is the best antimicrobial substance against E. coli

  15. Methodology The comparing of natural antibacterial substance on E. Coli Essential oil and antibiotic plant substance N=12 Aloe (anthraquinones) N=3 Garlic (allicin) N=3 Tea Tree oil N=3 Yuzu fruit oil N=3 Petri dish, E.coli, filter paper, glass spreader Disc diffusion assay method Millimeter SPSS ANOVA Test

  16. Protocols Paper disk with substance being tested Bacteria on agar Inhibited Zone(mm) Figure 12: Disk Diffusion method http://diverge.hunter.cuny.edu/~weigang/Images/20-17_diskdiffusion.jpg

  17. Protocols (cont) Figure 13: http://gsbs.utmb.edu/microbook/images/fig10_5.jpg

  18. Do-Ability • Not expensive • doesn’t require large amount of space • Some materials found in lab or supermarket • E. coli is needed as well as agar, aloe, tea tree, yuzu, and garlic • Natural antiseptic doesn’t have negative effect

  19. Budget

  20. Bibliography • Aiello, Castoki. Consumer Antibacterial Soaps: Effective or Just Risky?. The Infectious Disease Society of America Journal. Vol 10, p1058-1078, 2007 • Aljady, A. Biochemical Study on The Efficacy of Malaysian Honey on Inflicted Wounds: An Animal Model. Medical Journal of Islamic Academy of Sciences. Vol 13 #3. pg 125-132, 2000 • American Chemical Society. "Love That Garlic? Fresh May Be Healthier Than Bottled." ScienceDaily 12 June 2008. 7 May 2009 <http://www.sciencedaily.com­ /releases/2008/06/080609092230.htm>. • Amagase, H., Intake of garlic and its bioactive components. Nutritional Journal. Vol131(3S) pg 955S-962S, 2001 • "antiseptic." Grolier Multimedia Encyclopedia. 2009. Grolier Online. 3 May 2009 <http://gme.grolier.com/cgi-bin/article?assetid=0013590-0>. • Avallone, Eugene A., ed. "Antibiotic." McGraw-Hill Encyclopedia of Science and Technology. 8th ed. 20 vols. United States of America: Library of Congress, 1960. 756-67. • Brunner, Nasco. Do Bioactive Glasses Convey A Disinfecting Mechanism Beyond A Mere Increase in pH?. Internation Endodontic Journal, Vol 41(8) p670-678, 2008 • Carr, Anitra. Therapeutic Properties of New Zealand and Australian Tea Trees. New Zealand Journal, Vol 18#2, pg 47-51. 2009 • Chen, Tao. Triclocarban Enhances Testoterone Action: a New Type of Endocrine Disruptor?. Endocrinology journal. Vol 1. p 10-27, 2007 • Cuttle, Leila. The efficacy of Aloe vera, tea tree oil and saliva as fist aid treatment for partial thickness burn injuries. Elsevier Journal. Vol 1. pg 1176-1182. 2008 • Davis. D. "Antibacterial Chemical Disrupts Hormone Activities, Study Finds." ScienceDaily 8 December 2007. 3 May 2009 <http://www.sciencedaily.com­ /releases/2007/12/071207150713.htm>.

  21. Bibliography (cont) • Freeman, Frachesco. Garlic chemistry: stability of s-(2-propenyl)-2-propene-1-sulfinothioate (allicin) in blood, solvents, and simulated physiological fluids. J. Agric. Food Chem. Journal. Vol 43 pg2332-2338, 1995. • Fulder, Stephen. Garlic: nature's original remedy. 2nd ed. United States: Inner Traditions / Bear & Company, 2000. • Ingram, Cass. Tea Tree Oil: The Natural Antiseptic. 1st ed. United States: Knowledge House, 1999. • Koizum. Love That Garlic? Fresh May Be Healthier Than Bottled : Diallyl sulfides in garlic activate both TRPA1 and TRPV1. Biochemical and biophysical research communications journal. Vol 19. p929-934, 2009 • Lawson L.D, Characterization of the formation of allicin and other thiosulfinates from garlic. Planta Medical Journal. Vol 58. pg345-350, 1992. • Macpherson, Casey. The Pungency of Garlic: Actiation of TRPA1 and TRPV1 in Response to Allicin. Current Biology Journal. Vol 15, p929-934, 2005 • Nagane, N. Efficacy of Topical Honey Therapy Against Silver Sulphadizine Treatment in Burns; A biochemical study.Indian Journal of Clinical Biochemistry,Vol 19#2. pg 173-176. 2004 • Nakazono, G. Studies on Antimicrobial and Antioxidative Substance of Yuzu (Citrus junos hort. ex Tanaka) Seed. Journal of Biological Sciences. Vol 1. p 135-139, 2006 • Rusenova, Ishiwa. Antimicrobial Activities of Twelve Essential Oils Against Microorganisms of Veterinary Importance. Trankia Journal of Sciences. Vol 7. p37-43, 2009

  22. Bibliography (cont) • Shashikanth, K.N. Effect of feeding raw and boiled garlic (Allium sativum l.) on the growth, caecal microflora and serum proteins of albino rats. Nutrition Reports Int. Journal. Vol33(2) pg 313-319. 1986 • Society for General Microbiology. "Tea Tree Oil And Silver Together Make More Effective Antiseptics." ScienceDaily 6 April 2009. 3 May 2009 <http://www.sciencedaily.com­ /releases/2009/03/090329205453.htm>. • Tao. Cho. Chemical Composition of Essential Oil From The Peel of Satsuma Mandarin. Journal of Natural Sciences. Vol 7(9), p1261-1264,2008

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