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Simultaneous Measurement of Necrotic,

Simultaneous Measurement of Necrotic,. Apoptotic Dead and Live Cells. Necrotic/Apoptotic Dead. Treat Cells with DNAse to nick cells. Incorporate FITC-UTP with TdT. Apoptotic Live. Fix cells and permeabilize. Incorporate B-UTP with TdT then. PE-Avidin. SIMULTANEOUS EVALUATION OF

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Simultaneous Measurement of Necrotic,

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  1. Simultaneous Measurement of Necrotic, Apoptotic Dead and Live Cells Necrotic/Apoptotic Dead Treat Cells with DNAse to nick cells Incorporate FITC-UTP with TdT Apoptotic Live Fix cells and permeabilize Incorporate B-UTP with TdT then PE-Avidin

  2. SIMULTANEOUS EVALUATION OF DEAD, NECROTIC AND APOPTOTIC CELLS 0 1 2 3 4 10 10 10 10 10 0 256 512 768 1024 FITC UTP -> FSC -> TDT48015 TDT48015

  3. LIGHT SCATTER ANALYSIS OF DEAD CELLS AND VIABLE APOPTOTIC CELLS R4 R3 gated R1 A B PE-dUTP SSC R1 R2 Fl-12-dUTP FSC gated R2 gated R3 C D SSC SSC FSC FSC

  4. FOUR COLOR DETECTION OF SURFACE ANTIGENS, APOPTOSIS AND DEAD CELLS DEAD CELLS DEAD CELLS PE-CD19 APOPTOSIS TC-CD33 APOPTOSIS

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