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Experimental Evolution

Experimental Evolution. Silke & Elsa Hopkins Microbiology. Selection for fit types in a variable population Selection pressure: antibiotic Mutants survive Are Nal R mutants less fit in an antibiotic -free environment? Tradeoff?.

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Experimental Evolution

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  1. Experimental Evolution Silke & Elsa Hopkins Microbiology

  2. Selection for fit types in a variable population • Selection pressure: antibiotic • Mutants survive • AreNalRmutantsless fit in anantibiotic-free environment? Tradeoff?

  3. Select forNalRmutants (differentNal-Conc)correlation between selective pressure and number of mutants? • Nucleotide sequence of candidate genes  Mutations? • Recovery of fitness during many generations when selection removed? Duration?

  4. Suggestions: Maintain selective pressure for NalR Mutant up to “cage fight” ? Plate replicates and/or various timepoints

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