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Influence of Algae Treatment on Lagoon Odors. Personnel. Principal Investigator Stephen J. Reynolds, Ph.D., CIH Co-Investigators Tim Stanton, Ph.D. Brad Lester, M.S. Marc Cusanelli John Mehaffy, M.S. Regional Community Partners. Agsmart Inc. La Luna Dairy, Wellington, Colorado
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Personnel • Principal Investigator • Stephen J. Reynolds, Ph.D., CIH • Co-Investigators • Tim Stanton, Ph.D. • Brad Lester, M.S. • Marc Cusanelli • John Mehaffy, M.S.
Regional Community Partners • Agsmart Inc. • La Luna Dairy, Wellington, Colorado • Morwai Dairy, Hudson, Colorado • Weld County Commissioners • Cooperative Extension • CSU Center for Animal Agriculture and Community Enhancement
Specific Aims and Objectives • Work with community-based partners to conduct project and disseminate findings • Implement and evaluate the effectiveness of lagoon algae treatment on reduction of emissions and occupational exposures • Measure and quantify occupational exposures • Differences in dairy and feedlot size and manure handling practices • Evaluate the utility of the Cyranose 320 electronic nose for detection, identification, and quantification
Methods • Algae System – grown on-site in a greenhouse, added to manure by pump, raise dissolved oxygen to above 1.0 mg/ml • Total Particulate– 37-mm closed-faced cassette • Inhalable Particulate– IOM sampler with 25-mm filter and SKC Button sampler with 25-mm filter • Gas/Vapor – Sorbent tubes (charcoal and XAD-7) following NIOSH Analytical Methods
Methods • Hydrogen Sulfide– Jerome 631-X Hydrogen Sulfide Analyzer (Arizona Instruments) real-time measurement ranging from 3ppb to 50ppm • Ammonia– Dräger Pac III real-time measurement range from 0 to 300ppm • Bioaerosols– SKC Biosampler and Anderson Two-Stage Jet-to Agar Impactor (mesophilic and gram-negative bacteria, and fungi) • Odor and Constituent Gases– Scentometer and Cyranose 320 electronic nose
Methods • Endotoxin – Recombinant Factor C Endotoxin Assay (Biowhittaker) • Carbon Dioxide and Carbon Monoxide– Q-Trak (TSI Inc.) direct reading with datalogging • DataRam– particulate sampling in range from 0.01 to 100 mg/m3 • Grimm– real-time size selective evaluation of aerosol counts and mass, size range 0.35 to 20 µm, mass range 0 to 100 µg/L
Methods • Area Samples by Lagoons – Total and Inhalable Particulate and Endotoxin, Gases, Vapors, Bioaerosols, and Meterological data • Personal Samples – Total and Inhalable Particulate and Endotoxin for Milkers, Pushers, Feeder, Calf Feeders, Vet Techs, and General Maintenance Personnel
Algae System at a Colorado Dairy • 1500 cows • 60 acre site • flushes 250,000-400,000 gpd • recycles wastewater with fresh • odor issues with town residents • 8 acre lagoon • no land application
Colorado Dairy Set up system June 1, 2003 • GOALS: • REDUCE ODOR • GAIN COMMUNITY ACCEPTANCE
Colorado Dairy DISSOLVED OXYGEN (MG/L) MEASURED AT A DEPTH OF 6 FEET • DATA COLLECTED BY COLORADO STATE UNIVERSITY RESEARCH TEAM • CITY MAYOR & COUNCIL MEMBERS SUPPORTIVE OF AGSMART PROCESS • DAIRY ODOR COMPLAINTS DRASTICALLY REDUCED
Wastewater Analysis BOD5 10/01/04 04/28/05 09/06/05 Influent to Pond 12,640 mg/L 8,688 mg/L 3,924 mg/L Effluent to Barns 12,720 mg/L N/A 3,504 mg/L Pond Sample 12,580 mg/L 8,556 mg/L N/A Colorado Analytical Lab #04100111 #05042818 #05090637
Wastewater Analysis PO4 Wastewater Analysis—PO4 in mg/L 09/17/2003 1598 03/11/2004 1402 10/01/2004 648
Colorado Dairy H2S LEVELS MEASURED AROUND PERIMETER OF LAGOON DATE MEASURED READING 03/18/04 23 PPB 04/05/04 1-10 PPB
Traditional Mechanical Aeration System A Direct Comparison Of Aerobic Systems* 6-Tank System 139 hp 4-hp VS 556 Micro Diffusers 8-Acre Single Cell Lagoon With 28 Micro Diffusers 8-Acre Single Cell Lagoon With 556 Micro Diffusers Data Compiled By Colorado State University Installation Cost: Less Than $100,000.00 Installation Cost: Greater Than $140,000.00 Yearly Operation Cost: Less Than $36,000.00 Yearly Operation Cost: Greater Than $100,000.00 @ $.08 / KWH * TO OVERCOME13,000-16,000 MG/L BOD LOADING
Personal Sample Setup SKC Button Sampler
Scentometer Winter 05 Spring Summer Fall Winter 06
Hydrogen Sulfide Winter 05 Spring Summer Fall Winter 06
Ammonia Winter 05 Spring Summer Fall Winter 06
Total Particulate Lagoon Maint. Milkers
Total Endotoxin Lagoon Maint. Milkers
Inhalable Particulate Lagoon Calves Sick Cows Maint. Feeder Loader
Inhalable Endotoxin Lagoon Calves Sick Cows Maint. Feeder Loader
Questions? Nope, we have algae! Do you smell that?