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DNA Fingerprinting

DNA Fingerprinting. DNA forensic. Adalah metode analisis kejahatan forensik dengan menggunakan sampel DNA Contoh kasus : Bom Bali, JW Mariot , pembuktian jasad Nurdin M Top. Koleksi Spesimen Sampel. Sumber dapat diambil dr : - pakaian - rekatan amplop - putung rokok

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DNA Fingerprinting

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  1. DNA Fingerprinting

  2. DNA forensic • Adalahmetodeanalisiskejahatanforensikdenganmenggunakansampel DNA • Contohkasus: Bom Bali, JW Mariot, pembuktianjasadNurdin M Top

  3. KoleksiSpesimenSampel • Sumberdapatdiambildr: - pakaian - rekatanamplop - putungrokok - tepicangkir • Sampelygdiambildptberupa: darah, keringat, salivacairan semen, rambut, kuku, otot,dll

  4. Preparasisampel • Gunakansarungtangan disposable • Gunakaninstrumen disposable • Hindariberbicara, bersin, danbatuk pd saatpengambilansampel • Hidarimemegangbarangbukti • Pengeringansebelumpembungkusanbarangbukti • Hindarkandrsuhupanasdancahayamataharilangsung • Ditambahkanagen anti clotting (EDTA)M

  5. Ekstrak DNA • Dimurnikan dg larutan detergent (SDS/ fenol chloroform) untukmenghilangkan material selygtidakdiinginkan • Pengambilan DNA daridalamsel • Disentrifugasi (pemisahan)

  6. Analisis RFLP • Restriction Fragment Length Polymorphism (RFLP) • Ditambahkanenzimendonukleaserestriksiuntukmemotong DNA target pd sekuenstertentu. • Di elektroforesisuntukmemisahkanpotongan fragment DNA • Potongan DNA denganberatmolekulkecilakanlebihcepatbermigrasi (bp)

  7. Southern Blot • Setelahdielektroforesisfragmen pd gel agaroseditransferkemembrannitroselulosa • Cetakan pd nitroselulosa “fingerprint” • Diinkubasidenganditambahkanfragmen DNA radioaktif (probe) ygakanberhibridisasi dg sampel • Probe ygtidakberhibridisasiakandicucidihilangkan • Dilihatdibawahsinar X (autordiograph)

  8. PCR • Untukmengamplifikasi DNA sampelygjumlahnyasedikit. • DigunakanTaq Polymerase untukmembangunnukleotidabaru • Alat: thermal cycler

  9. VNTRs Variable Number Tandenm Repeats (VNTRs) Deretanbasaberulangpadaintron. VNTRs diwariskandrorangtua, ataukombinasidarikeduaorangtua. Biasadipakaiuntukidentifikasianggotakeluarga

  10. DNA mitokondria • Untukidentifikasihubunganantaraanakkandung – ibukandung • Me “match” kansidikjari DNA mitokondriadariduasampel

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