1 / 2

Lehrst uhl für Biotechnologie RWTH Aachen University Supervisors: Prof Dr. U. Schwaneberg

Internship: Directed evolution of Perhydrolase for improved stability in the presence of peracids and H 2 O 2. Lehrst uhl für Biotechnologie RWTH Aachen University Supervisors: Prof Dr. U. Schwaneberg Ljubica Vojcic. Techniques included in training. Molecular biology techniques:

nemo
Download Presentation

Lehrst uhl für Biotechnologie RWTH Aachen University Supervisors: Prof Dr. U. Schwaneberg

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript

  1. Internship: Directed evolution of Perhydrolase for improved stability in the presence of peracids and H2O2 Lehrstuhl für Biotechnologie RWTH Aachen University Supervisors: Prof Dr. U. Schwaneberg Ljubica Vojcic

  2. Techniques included in training • Molecular biology techniques: • PCR ampilification and purification of PCR product • Mutagenesis methods (epPCR, Site Saturation Mutagenesis/Site Directed Mutagenesis) • Restriction cloning of Perhydrolase gene • Megawhop cloning • Transformation of E. coli and B. subtilis strains • Biochemical methods: • Expression of Perhydrolase in B. subtilis • Screening –determination of proteolytic activity and finding improved variants for oxidative stability • Purification of Perhydrolase • Electrophoresis techniques – SDS electrophoresis and Experion system

More Related