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The Insecticide Resistance Section Of The PopBio Database Emmanuel Dialynas IMBB/FORTH, Greece 18 July 2013. The Process. Data collection & recording Submission - Curation Presentation. What can be recorded?. Ιnsecticide & concentration Synergist & concentration Assay method.
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The Insecticide Resistance Section Of The PopBio DatabaseEmmanuel DialynasIMBB/FORTH, Greece18 July 2013
The Process Data collection & recording Submission - Curation Presentation
What can be recorded? Ιnsecticide & concentration Synergist & concentration Assay method Investigator Food state Catch method Images/Photographs Anatomy parts Species identification method Collection period Sample sex Geolocation & coordinates Publication data Sample size Environmental conditions & factors
What results can be recorded? Enzymatic activity - Inhibition rate Permeability Mortality percentage KDT Protein absorbance Mutation frequency Lethal dose Resistance mechanism Disease transmission Variation Lethal concentration
Data collection and recording tools • Spreadsheet (Libre Office, Excel, Google Docs) • ISA-tools & ISA-tab. Desktop applications, standardized output. • Android mobile application and web interface in development.
Data collection and recording tools: Spreadsheets Most familiar. Easy to add extra fields. Data cannot be fully validated. Libre Office, Excel Work offline. Hard to collaborate. Frequent backup. Google Docs Integrated collaboration. Less frequent backups. Ontology assistant. Easier to get feedback/ support from Curator. Internet conn. required.
Data collection and recording tools: ISA-tools Form driven data entry Offline and online Most fields can be validated Ontology assistant Standardized output Frequent backups required Adding fields can be complicated No built in collaboration mechanism
Data collection and recording tools: Android application Recording data on the spot Process automation Date, time, location validation Built in image capturing Online/offline + + Date, time, coordinates PopBio
Data collection and recording tools: Web interface Online data entry Full data validation Ontology assistant Built in collaboration Offline
Data collection and recording tools: Web interface
Data searching & presenting • Geolocation • Year • Species • Insecticide • Catch method • Insecticide • Assay method • Result
Data searching & presenting Data provided by ANVR
Data searching & presenting All Anopheles arabiensis collections All collections in year 2000 Data provided by ANVR
Data searching & presenting All insecticides - all species - all years Data provided by ANVR
Data searching & presenting Mortality rates in permethrin assay >95% <=95% Data provided by ANVR
Data searching & presenting Lethal time 50 (LT50) in samples from Cameroon and Ghana
Giving Back To The Community • Training • Continuous support • IRbase installation at MRTC (Bamako, Mali) for WHO-AFRO
http://www.vectorbase.org info@vectorbase.org Special thanks to: WHO-AFRO, ANVR Janet Hemingway, Louise Kelly Hope, Clare Strode Hillary Ranson, Joseph Burrhani Funded by: