1 / 3

TOF-SIMS

Mineral content. TOF-SIMS. Caries activity. 4. POLMI & photo Micro-radiography. 7. 5. SEM (gold coated). Description. Teeth stored in NaCL and washed in etanol and then dried in air.

nirav
Download Presentation

TOF-SIMS

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript

  1. Mineral content • TOF-SIMS Caries activity 4. • POLMI & photo • Micro-radiography 7. 5. • SEM (gold coated)

  2. Description • Teeth stored in NaCL and washed in etanol and then dried in air. • Artificiell caries with lactate acid (Ten Cate et al 1996). Caries in a window of 4mm length, other surfaces covered with nailpolish. Section with caries stored in 37˚for 5 weeks. • Teeth cut, producing 2xsections 120μm and one bulk of the rest, with Leitz microtome saw • Polarized light microscopy, photo and microradiographs, for examination of mineralization of the caries lesion. • Section sputtercoated with gold for examination in SEM, where the topography is to be analyzed. • Section sputtercoated with carbon for examination in EDAX for carbon, oxygen, phosphorous and calcium - in and outside the lesion, making analyses possible intra and inter the different enamel specimen. • Enamel from the bulkspecimen of the slowest, medium and fastest caries activity (found out in 4.), polished with a diamond, for further mineral analysis as fluor and carbon.

  3. EDX Surface Caries lesion Enamel Dentin Circle area analysis (inter enamel analysis) Line-scan (intraenamel analysis)

More Related