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Identification of an AFLP marker for stem rust resistance in Avena strigosa. Taye Zegeye Daryl Somers Thomas Fetch Jr. Lakhdar Lamari. AAFC-CRC, Winnipeg. June 30, 2008. Materials and Methods. Two F 2 populations produced DNA extracted - Qiagen DNeasy 96 plant kit
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Identification of an AFLP marker for stem rust resistance in Avena strigosa Taye Zegeye Daryl Somers Thomas Fetch Jr. Lakhdar Lamari AAFC-CRC, Winnipeg June 30, 2008
Materials and Methods • Two F2 populations produced • DNA extracted - Qiagen DNeasy 96 plant kit • AFLP fingerprinting technique • Genomic DNA digested - PstI andMseI • BSA performed - 256PstI+MseIprimer pairs • PCR • ABI 3100 - Genetic Analyzer • A genetic map produced - JoinMap
Results F2 population from CN56979/CN57130 Primers: P-acg+M-cga RP SP M 4 82 56 90 400 370 350 340 300 250 200 160 150 135
Results - Mapping 0 PaatMccg15 2 PaaaMcga285 5 PaaaMcga 8 PaggMcaa155 15 PaggMcaa205 16 PaggMcaa190 22 PaccMcgg230 28 PacgMcga370 37 Sr_57130 62 PaagMctc140 63 PaagMctc130 64 PaaaMcga330 66 PaagMctc110 71 PacgMcga120