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The Revolutionary Search for Alarin

The Revolutionary Search for Alarin. How Alarin and its Relation to GALP May Impact Society. Paulina Phomvongsa Dr. Fraley’s Lab. Framework. Overview What is GALP? What are the effects? How is alarin related to GALP? Why is this significant? The Research Aspect Hypothesis

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The Revolutionary Search for Alarin

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  1. The Revolutionary Search for Alarin How Alarin and its Relation to GALP May Impact Society Paulina Phomvongsa Dr. Fraley’s Lab

  2. Framework • Overview • What is GALP? • What are the effects? • How is alarin related to GALP? • Why is this significant? • The Research Aspect • Hypothesis • Methods & Techniques • Observations http://www.engelhard.com/images/Application/low%20res%20flasks%204.jpg

  3. What is GALP? • Stands for Galanin-Like Peptide • Neuropeptide composed of 60 amino acids • Member of the galantide family • 2 members: galanin & GALP • Aren’t found on same chromosomes • Have different effects in the brain • Found in neurons located in Arcuate nucleus of hypothalamus • Regulated by leptin and insulin

  4. What are the Effects? • Stimulates the hypothlamic-pituitary-gondal axis • Effects the release of reproductive hormones • Body weight and feeding behavior  GALP production   stimulates reproduction • Diseases affiliated with GALP: anorexia nervosa/exercise-induced anorexia & Type I diabetes

  5. Anorexia Diabetes http://www.lostiempos.com/lecturas/07-01-07/fotos/anorexia.jpg http://www.faqs.org/health/images/uchr_07_img0705.jpg

  6. How is Alarin Related to GALP? • GALP discovered in 1998 • Is an alternate transcript of GALP gene • Found in brain tumor cells: neuroblastomas • Both found on the same gene • GALP has 5 exons, alarin is missing exon 3 and therefore has 4 exons

  7. What We’re Looking For • Can the alarin protein be isolated in the normal brain? • What does it control? • What are the effects?

  8. Hypotheses • Alarin has functions similar to GALP’s • Regulates body weight, food intake and reproductive behaviors

  9. Methods & Techniques • Lateral Ventricle Cannulations • In Vivo injections • PCR • Look for alarin cDNA • ICC • Look for cell bodies where alarin is produced and where their axons project

  10. Observations • Alarin increases hunger • Opposite effect of GALP • Rats injected with alarin display tranquil behavior • Rats injected with alarin were found laying down more often than normal

  11. Summary • Alarin exists naturally in mammalian brain • Although alarin increases food intake, its action appears to be very different from GALP

  12. Acknowledgements • Dr. Gregory. S. Fraley • Co-Workers • Hope College Biology Department • Hope College REACH Program • NIH

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