1 / 8

Blood samples

Blood samples. Kari Kuulasmaa Luxembourg 9-11 April 2008. Uses of blood samples. Lipid measurements Total cholesterol HDL-cholesterol Glucose Fatty acids and lipoproteins Biomarkers E.g. Refl. nutrition (vitamins, flavonoids..) Antibodies Markers of infection and inflammation DNA.

oren
Download Presentation

Blood samples

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript

  1. Blood samples Kari Kuulasmaa Luxembourg 9-11 April 2008

  2. Uses of blood samples • Lipid measurements • Total cholesterol • HDL-cholesterol • Glucose • Fatty acids and lipoproteins • Biomarkers • E.g. Refl. nutrition (vitamins, flavonoids..) • Antibodies • Markers of infection and inflammation • DNA

  3. Blood collection • Fasting • Not required for cholesterols • 8 h (overnight) for glucose • By certified phlebotomist • Subject in sitting position • Minimal use of tourniquet

  4. Cholesterol • Serum preferred • Clotting in room temperature for 30-60 min • Centrifuging within an hour • Analysis preferably on the day of collection • or on refrigerated samples within 3 days • or frozen samples (max 6 months at -20, longer at -70)

  5. Glucose • Tubes with citrate and fluoride • Centrifuge within an hour • Analysis preferably on the day of collection • or on refrigerated samples within 3 days • or frozen samples (max 6 months at -20, longer at -70)

  6. Change (%) of glucose concentration in whole blood samples Uchida et al. Clinica Chimica Acta 1988;172:101-108

  7. Change (%) of glucose concentration in whole blood samples Citrate-fluoride mix NaF/K-oxsalate NaF/EDTA Li-hep Time (h) Väisänen et al. Kliin Lab 2008; 3-4

  8. Laboratory procedures • Use accredited laboratory • Good direct enzymatic methods are available • CDC has a certification program for clinical diagnostic products for cholesterols • External quality control needs to be set up

More Related