1 / 21

Potential Algal Biofuel Applications in West Virginia

Potential Algal Biofuel Applications in West Virginia. STaR Symposium October 23, 2013. Derrick R. J. Kolling Department of Chemistry. Talk Overview. Biofuels overview Why do we need biofuels? The light reactions of photosynthesis 2H 2 O + 4h   4H + + 4e - + O 2 Tools of the trade

orenda
Download Presentation

Potential Algal Biofuel Applications in West Virginia

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript

  1. Potential Algal Biofuel Applications in West Virginia STaR Symposium October 23, 2013 Derrick R. J. Kolling Department of Chemistry

  2. Talk Overview • Biofuels overview • Why do we need biofuels? • The light reactions of photosynthesis • 2H2O + 4h 4H+ + 4e- + O2 • Tools of the trade • O2 electrode • Fluorometer • EPR spectrometer • GC-MS • Research projects • OEC photoassembly • Temperature dependence of molecular photosynthesis • Algal lipidomics • Biofuels from invasive algal species

  3. Renewable Energy futurefarmers.com/survey/algae epmb.berkeley.edu Biofuels Solar power Wind power Tidal power Hydropower Geothermal

  4. Dismukes et al. (2008)

  5. lipid biodiesel protein animal feed carbohydrate anaerobic O2 ATP Fermentation CO2, organic acids H2 ethanol Photosynthesis Biomass CO2 Carbon Fixation Lipid Synthesis Protein Synthesis Electrons (as NADH, NADPH, Reduced Ferredoxin) e- ATP O2 H2O Source: Damian Carrieri H2O

  6. Light Reactions Dismantling photosynthesis from the organism to the molecules….

  7. Umena et al. (2011) Nature Light Reactions …from the molecular to the atomic level Barber & Iwata (2004) Science

  8. Research Tools • Fluorescence • Oximetry • Electron Paramagnetic Resonance • GC-MS

  9. Temperature dependence of oxygen evolution PSII PSII w/DCBQ PSII normalized OEC Photoassembly

  10. Temperature dependence of oxygen evolution vs. photoassembly Intact PSII Photoassembled PSII at 28°C OEC Photoassembly -Share a rate-limiting step

  11. OEC Photoassembly -Protease activity explains dip

  12. OEC Photoassembly Findings • Oxygen evolution and OEC photoassembly share a 28°C maximum • OEC photoassembly and PSII oxygen evolution appear to share a rate-limiting step • Thermal inactivation of PSII and apo-PSII occurs at temperatures greater than 28°C • Thermal inactivation is partially due to protease activity (Deg and/or FtsH?)

  13. Artificial Leaf www3.imperial.ac.uk www.ruhr-uni-bochum.de/h2design/profile/main.html OEC Photoassembly Application Bioinspired/biomimicked System

  14. Algal Lipidomics Comparison of lipid accumulation in photomixotrophically and heterotrophically grown Chlorella vulgaris cultures

  15. Algal Lipidomics Measured: -[Chl a] -turbidity -dry weight -lipid dry weight Culture 1 2 3 4 5 6 [ [ [ [ [ [ [ [ [ 2 4 0 1 3 5 6 7 8

  16. Algal Lipidomics Chl a Expression Cell Density750nm Derrick R. J. Kolling October 23, 2013

  17. Algal Lipidomics Dry Weight Growth Curves and Corresponding Dry Lipid Weights C 16: palmitic acid C 18: stearic acid C 18:1(n-9): oleic acid C 18:2(n-6): linoleic acid

  18. Algal Lipidomics Findings • Photomixotrophically grown cells produce 2X as many lipids as do heterotrophically grown cells • Photomixotrophically grown cells reach stationary phase and higher biomass sooner • Cells produce palmitic, stearic, oleic, and linoleic acids

  19. Algal Lipidomics Application Chisti (2007) Biotech. Advances

  20. Acknowledgements Acknowledgements • Temperature Dependence of PSII • -James Board, Hope Cook, Matt Thompson, Shane Kagen, Jordan Hilgeford, Justin Erwin • OEC photoassembly • -James Board, Hope Cook, Ben Blodgett, Matt Thompson, Shane Kagen, Jordan Hilgeford, Chase Turner, Ben Weiner • Algal lipidomics • -Ben Woodworth, Tony Stephenson, Rebecca Mead, Courtney Nichols, Prof. Leslie Frost (MU) • Bioethanol from invasive algal species • -Kevin Militello, ShaheedElhamdani Funding This material is based upon work supported by the National Science Foundation under Cooperative Agreement Award number EPS-1003907 and CHE-1229498.

More Related