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Beyond pGLO

Beyond pGLO. Genetic Engineering and Stem Cells. Restriction enzymes: Have sticky ends or overhangs of DNA. This helps to “glue” in the gene of interest in the right direction. Must find a plasmid with the appropriate sticky ends that match your gene. Sticky Ends. Polymerase Chain Reaction

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Beyond pGLO

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  1. Beyond pGLO Genetic Engineering and Stem Cells

  2. Restriction enzymes: • Have sticky ends or overhangs of DNA. • This helps to “glue” in the gene of interest in the right direction. • Must find a plasmid with the appropriate sticky ends that match your gene. Sticky Ends

  3. Polymerase Chain Reaction • Taq polymerase • Nucleotides • Thermocycler • http://www.dnalc.org/resources/animations/pcr.htm PCR

  4. iPS stem Cells

  5. Model for gene therapy

  6. How might cell-based delivery avoid problems associated with direct delivery

  7. Pre-implantation genetic diagnosis

  8. Gel Electrophoresis: • http://www.dnalc.org/resources/animations/gelelectrophoresis.html • Gene Chip: • http://www.sumanasinc.com/webcontent/animations/content/dnachips2.html • How could gene chip technology transform medicine as we know it? How do you tell a cell has a certain gene?

  9. Learn how a mouse is cloned. • http://learn.genetics.utah.edu/content/cloning/clickandclone/ • Many copies of identical mice are cloned from mouse embryos. You can have essentially 1000 identical twins with the same genetics. • This has been used in science for many years. What is cloning?

  10. Epigenetic patterns already in place (how is DNA tagged to turn genes on and off?) • Transplantation process is costly. • Cells are genetically “older”. Telomerase has been deactivated. Problems with cloning from adult cells

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