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14. Spectroscopy of Cobalt (II) Ion. Goal; Determine concentration (in Molarity) of unknown cobalt solution using spectroscopy and a calibration graph. Measure how much Electromagnetic radiation Is absorbed by sample. UV/vis . Beer’s Law A= Absorbance e = proportionality constant
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14. Spectroscopy of Cobalt (II) Ion • Goal; Determine concentration (in Molarity) of unknown cobalt solution using spectroscopy and a calibration graph. Measure how much Electromagnetic radiation Is absorbed by sample UV/vis Beer’s Law A= Absorbance e = proportionality constant b= path length c = Concentration in Molarity A=ebc
Determine optimum wavelength, , to maximize absorption of Co(NO3)2 (aq) • Measure absorbance at different wavelengths. Run second part of experiment at which has maximum absorbance. Absorption Wavelength, l
Make Calibration Graph and Determine Concentration from Absorbance x x Absorbance, A x x Concentration, M
Determine how many grams of Co(NO3)2*6H2O is needed to make 50.0 ml of a 0.150 M solution. MM (Co(NO3)2*6H2O) = 291.03 g/mole Moles = mass/ Molar Mass Molarity = moles solute/ Liter solution
Determine Molarity of a solution made by taking 2.00 ml of a 0.150 M Co(NO3)2*6H2O solution and adding 3.00 ml water to get a total volume of 5.00 ml. (MV)Before dilution = (MV) After dilution