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Bacteriology

Bacteriology. Genetic Recombination. Transformation Bacterium absorbs DNA from environment. Plasmid with ampicillin resistance. Genetic Recombination. Conjugation Pilus extends between cells Replica of a plasmid is donated . Plasmid with ampicillin resistance.

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Bacteriology

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  1. Bacteriology

  2. Genetic Recombination • Transformation • Bacterium absorbs DNA from environment Plasmid with ampicillin resistance

  3. Genetic Recombination • Conjugation • Pilus extends between cells • Replica of a plasmid is donated Plasmid with ampicillin resistance Plasmid with ampicillin resistance

  4. Genetic Recombination • Transduction • Virus carries host DNA to next host Gene with ampicillin resistance

  5. Significance of Bacteria Why do we care?

  6. Pathology Tuberculosis

  7. Fermented Foods

  8. Medical Research • Medications • Vaccines • Restriction enzymes

  9. Environmental Issues • Decomposition • Ethanol • Insecticides • Nitrogen fixation

  10. Oxygen Tolerance • Obligate Anaerobes • Facultative Anaerobes • Obligate Aerobes

  11. Bacterial Size

  12. Outer Coverings • Cell Membrane • Wall (peptidoglycan) • Gram+ • Simple, thick wall • Gram- • Thin wall • Outer membrane • Capsule • Adheres cell to food • Disguises against host immune system

  13. Identification: Gram Staining Crystal Violet Iodine Ethanol Safranin + -

  14. Identification: Morphology Coccus Bacillus Spiral

  15. Identification: Morphology • Coccus • Diplococcus • Streptococcus • Staphylococcus

  16. A C B F E G D I J H

  17. Endospores • DNA, ribosomes, and some cytoplasm encased • Defense against temperature, pH, chemicals, and aridity

  18. Review • How might bacteria cause disease? • How can one determine which bacterium is making a person sick? • How can bacteria pick up new abilities?

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