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Nonylphenol Degradation During Wastewater Treatment - Importance of Nitrifying Bacteria

Nonylphenol Degradation During Wastewater Treatment - Importance of Nitrifying Bacteria. Presented By: Natasha Sinha. Comparison of 4 Compounds: Compound (Use and Effect) C MAX (µg/L) Days to Med Dose Caffeine (stimulant) 5.7 5.7 x 10 3 (Excedrin Migraine)

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Nonylphenol Degradation During Wastewater Treatment - Importance of Nitrifying Bacteria

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  1. Nonylphenol Degradation During Wastewater Treatment - Importance of Nitrifying Bacteria Presented By: Natasha Sinha

  2. Comparison of 4 Compounds: • Compound (Use and Effect)CMAX (µg/L)Days to Med Dose • Caffeine (stimulant) 5.7 5.7 x 103 (Excedrin Migraine) • Ibuprofen (anti-inflammatory) 1.0 105 (Motrin) • 17α-ethinyl estradiol (oral contraceptive) 0.27 37 (Alesse-21) • 17β-estradiol (vertebrate hormone) 0.093 (Phoenix) 5.3 x 103 (Activella) • 4-nonylphenol (surfactant metabolite) 40 (Tucson) 4.6 x 10-4 as estrogenic E2, EE2 Motivation: USGS Surface Water Reconnaissance(Kolpin et al., 2002) Sampling regions for the USGS Reconnaissance of US surface water

  3. Secondary Effluent Chromotograph

  4. Further Motivation:Intersex Characteristics in Fish Populations These data represent the frequency of intersex characteristics of fish taken above and below wastewater treatment discharges

  5. Known Estrogenic Compounds in Wastewater

  6. Conventional Wastewater Treatment Processes

  7. Removal of Estrogens in Arizona Plants

  8. Background on Ammonia Oxidizing Bacteria • NH4+ NH2OH NO2 O2 AMO (ammonia monoxygenase) AMO is a non-selective monoxygenase • Central Hypothesis: Estrogen removal is due to AMO activity in culture.

  9. Lab Setup Synthetic Wastewater Control Unit Clarified Effluent Reactor Variable: Sludge Age

  10. Experimental Procedure Part 1 • Step 1: Look for NH4+ stimulation of O2 use • Determine whether ammonia oxidizing bacteria are present.

  11. Result (1) OUR: 0.00054 OUR:0.00059 OUR: 0.00025 OUR: Oxygen Utilization Rate

  12. Was Nonylphenol Degraded? Measurements of nonylphenol degradation were taken after 24 hours and 48 hours using a C-18 procedure and Gas Chromatography

  13. Removal of Nonylphenol + Data

  14. To Come • Repeat with longer sludge retention time • Compare to previous results • Supplement with effluent NH4+ data

  15. Acknowledgements Dr. Robert Arnold Dave Newman Bingfeng Dong Dustin Brown & Arthur Ho

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