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Science as Inquiry Initiative

Enhance undergraduates' learning through a pilot genome lab project, focusing on massively parallel DNA sequencing technologies and genomic analysis from single living neurons. The initiative promotes student-led discoveries and scientific literacy.

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Science as Inquiry Initiative

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  1. Science as Inquiry Initiative • Undergraduates make new discoveries, rather than just learn what already is known • Pilot project • to develop, implement and assess genome lab curriculum [including wet lab & computer lab, majors & non majors]

  2. JP Sulzberger MD Genome Center • Massively parallel DNA sequencing • Genomes and Undergraduates Initiative

  3. JD Sulzberger MD Columbia Genome Center Center for Excellence in Genomic Science • Development of • new genomic technologies for massively parallel DNA sequencing and • large-scale gene expression analysis from single living neurons, axons, and synapses • Application of these technologies to study neural functions. • Molecular Libraries Screening Center • National Center • High-throughput cell biology Rothman Laboratory Applications of Massively Parallel DNA sequencing 1 = >100 Educational Outreach

  4. Wednesday Afternoon • 12:30-1:30 Lunch Application of New DNA Sequencing Technologies • for the Study of Epigenetic Abnormalities in Breast Cancer John Edwards • 1:30-3:30 • Tour Columbia Genome Sequencing and Chemical Biology Lab & • Molecular Libraries Screening Network Lab

  5. What’s unique about our Center? Different Communities have different sequencing priorities Focus on communities not served by current sequencing infrastructure. Which genomes would you sequence first?

  6. Genome Sequencing

  7. Eliminate expensive BAC cloning infrastructure • [a major economy of scale that favored large highly automated sequencing centers] • PCR in emulsion or on bead • [Eventually, eliminate PCR…] • Highly Parallel Sequencing by Synthesis • Detect incoming bases in real time during synthesis A T T G G G C A T A T T C G T A A C C C G

  8. Massively Parallel Sequencing by Synthesis Solexa/Illumina Technology Note: PCR done in situ without resource-intensive BAC-cloning infrastructure

  9. 40 million sequences in parallel

  10. Genome Sequencing PrioritiesNothing in biology makes sense except in the light of evolution --Dobzhansky Evo - devo emphasis • Key steps on journey of evolution • Profound biochemical relationships of all life on earth • Comprehend the building up of biochemical interactions and regulation http://tolweb.org/tree/

  11. The laboratory is the course • Select, research-oriented faculty • Students make original discoveries • 15 partner colleges* • Top tier liberal arts colleges • Diversity: Women, Minorities, first in family to attend college • Geographic diversity to seed national network • National Genomics Pedagogy Website [SERC] *organizational meeting supported by Schering-Plough Foundation http://www.eeob.iastate.edu/faculty/DrewesC/htdocs/Q-lumbriculus.htm

  12. Teach science as it is practiced • Increase the pool of talented scientists • Retain students who plan to major in science • “Lure” students into more advanced courses • Reach out to under-represented groups • Increase scientific literacy (focus on genomics) • Support for science • Conversant with scientific questions [public policy, personal medical decisions]

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