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EXPERIMENT # 3

EXPERIMENT # 3. SMEAR PREPARATION SIMPLE STAIN GRAM STAIN. Work in groups of two. . epidermidis. Dispose extra stain inside the plastic bottle in the sinks. 2. +. +. +. _. _ _. _ _. _ _. _ _. SIMPLE STAIN (w/ Methylene blue). Work in groups of two.

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EXPERIMENT # 3

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  1. EXPERIMENT # 3 SMEAR PREPARATION SIMPLE STAIN GRAM STAIN

  2. Work in groups of two .epidermidis Dispose extra stain inside the plastic bottle in the sinks 2

  3. + + + _ _ _ _ _ _ _ _ _

  4. SIMPLE STAIN (w/ Methylene blue)

  5. Work in groups of two III. Gram Stain: Differential stain to distinguish between Gram(+) and Gram (-) Use the remaining smears of each bacterium you have already prepared Cover each smear w/ crystal violet for 1 min Grasp the slides w. a slide holder and rinse w/ water Cover the smears w/. iodine for 1 min Rinse w/ water Decolorize w/ ethanol for 2-3 seconds. ONE SLIDE AT A TIME Rinse w/ water Cover slides w/ the counter stain: safranin for 1 min Rinse w/ water Blot w/ bibulous paper, don't rub or wipe Observe under immersion oil Dispose everything in its place and clean Dispose extra stain inside the plastic bottle in the sinks

  6. 1. Primary stain: Crystal Violet + 2. Iodine - - - - - - GRAM + 3. Ethanol: Decolorizer 1. Primary stain: Crystal Violet + 2. Iodine 3. Ethanol: Decolorizer - - - - - - GRAM -

  7. 1. Primary stain: Crystal Violet + 2. Iodine - - - - - - GRAM + 3. Ethanol 1. Primary stain: Crystal Violet + 2. Iodine 3. Ethanol - - - - - - GRAM -

  8. 1. Primary stain: Crystal Violet + 2. Iodine - - - - - - GRAM + 3. Ethanol 1. Primary stain: Crystal Violet + 2. Iodine 3. Ethanol - - - - - - GRAM -

  9. 1. Primary stain: Crystal Violet + 2. Iodine - - - - - - GRAM + 3. Ethanol 4. Safranin + 1. Primary stain: Crystal Violet + 2. Iodine 3. Ethanol - - - - - - 4. Safranin + GRAM -

  10. 1. Primary stain: Crystal Violet + 2. Iodine - - - - - - GRAM + 3. Ethanol 4. Safranin + 1. Primary stain: Crystal Violet + 2. Iodine 3. Ethanol - - - - - - 4. Safranin + GRAM -

  11. GRAM STAIN GRAM (+) (Staph epidermidis) GRAM(-) E. coli

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