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Microscopy Lab

Microscopy Lab. 13 November 2002. Microscope Basics. There are 2 lenses that contribute to total magnification – the ocular (the one you put your eye on) and the objective (the lenses that are on a rotating disk). Total magnification is the product of these 2 lenses.

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Microscopy Lab

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  1. Microscopy Lab 13 November 2002

  2. Microscope Basics • There are 2 lenses that contribute to total magnification – the ocular (the one you put your eye on) and the objective (the lenses that are on a rotating disk). Total magnification is the product of these 2 lenses. • Always start with the lowest magnification (the smallest objective lens). Find some objects, center and focus, then increase magnification. • At the highest magnification (usually 40X objective lenses): • Be careful swinging the lens into place. Do it slowly and carefully. • At this magnification, only use the fine focus – never the coarse focus.

  3. Assignment – To Be Done In Your Lab Notebook • Sketch the following organisms. Label the organelles that you see (typically you will only be able to see cell membranes, cell walls, cytoplasm and the nucleus). These don’t have to be detailed (you aren’t being graded on artistic ability), but they do need to reflect an honest effort to examine and diagram cells. Organisms: • Bacteria - Cheek cell (your own) • Animal cell (prepared slide) - Live onion cell • Onion root tip

  4. Preparing Cells • Cheek Cells: • Use a toothpick and scrape the inside of your cheek several time. Rub the toothpick on a slide. Add a small drop of Iodine and mix your solution with the toothpick. Add a coverslip a observe. • Onion Cells: • Peel away a thin layer of onion. Lay it flat on the slide, add a small drop of Iodine and a coverslip. Observe.

  5. Instructions • Draw a circle about 2” in diameter. Your diagram should fill this circle. • Label the diagram; what it is, what the organelles are and anything relevant. • Indicate the magnification

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