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Chapter 10, part B

Chapter 10, part B. Classification of Microorganisms. Numerical Identification. Figure 10.9. Serology. Combine known antiserum + unknown bacterium Slide agglutination ELISA Enzyme linked immunosorbent assay Western blot Looking for different what?. Figure 10.10. Western Blot.

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Chapter 10, part B

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  1. Chapter 10, part B Classification of Microorganisms

  2. Numerical Identification Figure 10.9

  3. Serology • Combine known antiserum + unknown bacterium • Slide agglutination • ELISA • Enzyme linked immunosorbent assay • Western blot • Looking for different what? Figure 10.10

  4. Western Blot Figure 10.12

  5. Phage Typing Figure 10.13

  6. Flow Cytometry Uses • Differences in electrical conductivity between species • Fluorescence of some species • Cells selectively stained with antibody + fluorescent dye Figure 18.11

  7. Genetics • DNA base composition • Guanine + cytosine moles% (GC) • DNA fingerprinting • Electrophoresis of restriction enzyme digests RFLP • rRNA sequencing • Polymerase Chain Reaction (PCR) Figure 10.14

  8. Nucleic Acid Hybridization Figure 10.15

  9. Nucleic Acid Hybridization: DNA probe Figure 10.16

  10. Nucleic Acid Hybridization: DNA chip Figure 10.17

  11. Figure 10.5

  12. Dichotomous Key

  13. Cladogram Figure 10.18.1

  14. Cladogram Figure 10.18.2

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