1 / 5

DMS

A. HIV-1 -AUG1. DMS. CMCT. Control. V1. +Mg 2+. +Mg 2+. -Mg 2+. -Mg 2+. C. T. A. G. G 640. G 650. A 660. A 665. A 670. A 675. C. T. A. G. 1. 2. 3. 4. 5. 6. 7. B. HIV-1 AUG 1. DMS. CMCT. V1. Control. +Mg 2+. +Mg 2+. -Mg 2+. -Mg 2+. G. C. T. A. A 500.

remedy
Download Presentation

DMS

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript

  1. A HIV-1 -AUG1 DMS CMCT Control V1 +Mg2+ +Mg2+ -Mg2+ -Mg2+ C T A G G640 G650 A660 A665 A670 A675 C T A G 1 2 3 4 5 6 7

  2. B HIV-1 AUG1 DMS CMCT V1 Control +Mg2+ +Mg2+ -Mg2+ -Mg2+ G C T A A500 C510 G520 C525 A530 C535 C T A G 1 2 3 4 5 6 7

  3. C HIV-1-AUG1 DMS CMCT V1 Control +Mg2+ +Mg2+ -Mg2+ -Mg2+ G C T A G400 A410 U420 A430 A440 A450 C T A G 1 2 3 4 5 6 7

  4. D SIVMAC-AUG1 DMS CMCT CONTROL V1 +Mg2+ +Mg2+ -Mg2+ -Mg2+ C T A G C880 G890 A895 A900 C T A G 1 2 3 4 5 6 7

  5. S3 : Examples of a typical probing experiments. Probing experiments were performed as described in material and methods. RNAs were reverse sequenced with reverse transcriptase (lanes CTAG), and a mock-treated RNA was transcribed in standard RT buffer to monitor RNA specific RT stops (lane 1 - control). RNAse V1 probing revealing paired positions was conducted in native conditions only because its activity requires Mg2+ ions (lane 2, 0.01u; lane 3, 0.1u). DMS and CMCT probing were performed both in absence (lanes 4 and 6 respectively) and presence of Mg2+ ions (lanes 5 and 7 respectively). Hallmarks are indicated on the left side of the gel. A - HIV-1 region from 630 to 680 B - HIV-1 region from 500 to 535 C – HIV-1 region from 400 to 460 D – SIVMAC region from 880 to 905

More Related