Tim Tripp Director, Biotechnology Resource Center 4/17/2013

1. Tim Tripp Director, Biotechnology Resource Center 4/17/2013

2. Outline The Biotechnology Institute General introduction Faculty The Biotechnology Resource Center Services Equipment Training

3. Provides advanced research, training, support, and university-industry interaction in biotechnology.

4. 29 years of Progress in Biotechnology Established in 1983, the BioTechnology Institute is a joint venture of the College of Biological Sciences and the College of Science and Engineering.

5. The BioTechnology Institute Continues to be a Cornerstone of University-Industry Interaction in the LifeSciences Renewed support from the University of Minnesota Continuous improvement and transformation

6. The BioTechnology Institute faculty represent four colleges at the University of Minnesota: COLLEGES • The College of Biological Sciences (CBS) • The College of Science and Engineering (CSE) • The Academic Health Center (AHC) • The College of Food, Agricultural, and Natural Resource Sciences (CFANS)

7. DEPARTMENTS The BioTechnology Institute draws faculty from the departments of: • Biochemistry Molecular Biology and Biophysics • Bioproducts & Biosystems Eng. • Center for Drug Design • Chemistry • Chemical Engineering and Materials Science • Civil Engineering • Ecology, Evolution, & Behavior • Genetics, Cell Biology, and Development • Mechanical Engineering • Microbiology • Plant Biology • Soil, Water & Climate

8. BioTechnology Institute faculty have broad expertise in the areas of: • Biocatalysis • Metabolic engineering/ microbial physiology • Combinatorial biology • Synthetic ecology • Population dynamics • Molecular Biology • Proteomics, and • Focused expertise in defined areas such as bioremediation, biomaterials, biosensors, and bioinformatics. FACULTY Michael Sadowsky Director Daniel Bond Robert Brooker Antony Dean Mark Distefano Jeffrey Gralnick Wei-Shou Hu Romas Kazlauskas Yiannis Kaznessis Arkady Khodursky C. Schmidt-Dannert Burckhard Seelig Friedrich Srienc Gary Dunny Michael Travisano Lawrence Wackett Ping Wang

9. The BioTechnology Institute faculty have broad expertise in the areas of: • Bringing together this expertise to expand knowledge in new and developing fields of commercially relevant life sciences. • Finding new applications for existing technology Alptekin Aksan Brett Barney Raymond Hozalski Christine Salomon George Karypis Timothy LaPara Igor Libourel Paige Novak Gary Muehlbauer Kechun Zhang Bo Hu Fransico Diez-Gonzalez

10. BTI faculty conduct research • over a broad spectrum of • disciplines, including: • Biocatalysis • Metabolic pathway engineering • Genetics and cell biology • Functional genomics • Biodegradation of hazardous materials • Molecular evolution • Biological diversity • Green chemistry • Protein engineering • Development of biofuels and biopolymers from renewable resources. RESEARCH

11. Important aspects of the BioTechnology Institute include: • The Biocatalysis Initiative - Synthetic Ecology • Graduate Program in Microbial Engineering • Scientist Exchange Program • Biotechnology NIH Training Grant INITIATIVES AND PROGRAMS

12. The BioTechnology Institute is a multidisciplinary and multi-departmental institute guided by three core values: • Conduct innovative, relevant research • Provide professional training • Serve as a resource for the University and industry CORE VALUES

13. Serves as a Resource FACILITIES & SERVICES • The BioTechnology Institute has invested significantly in facilities with updated equipment. • Facilities and services available to students and researchers include the: • The Biotechnology Resource Center • Pilot plant • Development lab • High Throughput Biological Analysis Facility The BRC

14. Biotechnology Resource Center A service provided by the Biotechnology Institute • Facility • The 5000-square-foot facility is located on the University of Minnesota’s St. Paul campus. • Equipment includes: • 14 fermenters with volumes of 6L, 10L, 20L, 75L, 300L, and 550L • 5 x 20L light-ring photobioreactors • batch and continuous-flow centrifuges • microfluidizer, high-pressure homogenizer, & French press • bench- to pilot-scale ultra-filtration units • bench- to pilot-scale chromatography • freeze dryer Fermentation/Cell Growth • Cultivation of bacteria, yeast & algae • Process development & optimization • Pilot-scale production Recombinant Protein Expression • E. coli & other bacterial expression systems • Pichia pastoris & S. cerevisiae Downstream Processing • Recovery and purification of proteins & small molecules GMP Capabilities • Fully established quality system • Production of Phase I and Phase II clinical trial material

15. The Biotechnology Resource Center Services: • Molecular biology (cloning, site-directed mutagenesis, expression vector construction, sequence verification) • Recombinant protein expression(e.g. E. coli, Pichia pastoris) • Fermentation process development& scale-up(bacteria, yeast & fungi) • Animal Cell Culture • Downstream processing (centrifugation, cell breakage, ultra- & micro-filtration, chromatography, freeze drying)

16. Customers • Ajinomoto • Aldeveron • Alvine Pharmaceuticals • Monsanto

17. Training Through the BRC • MicE 5355 • Week long lab course • MT 3141 • Half the semester in the lab • Custom programs • Multiple proposals

18. The Next Generation of Biotechnologists • Work Study Program for undergrads • Worthington Middle School 8th grade Science Club

19. Partners • Biomedical Genomics Center • Center for Mass Spectrometry • Proteomics Analysis Core • Imaging Center

20. Biomedical Genomics Center Services & Instrumentation : • DNA sequencing ABI PRISM™ 3730xl DNA Analyzer , Illumina GAIIx and HiSeq 2000, Roche GS FLX (454 Sequencing) • Single-nucleotide polymorphism (SNP) analysis & mutation detection • Real Time PCR (ABI 7700) • Micro/Macro array spotting and scanning (Biorobotics Total Array System, Packard Scanner, Affymetrix system) • RNA analysis (Agilent Bioanalyzer 2100) • Plasmid preparations (Qiagen 8000 robot, Hydra liquid handlers)

21. Center for Mass Spectrometry in the Life Sciences Services: • Identification, and quantification of proteins and small molecules • Protein & peptide sequencing • Identification of posttranslational modification of proteins Instrumentation: • 2x Thermo LC-Q w/ APCI & ESI • 1x Thermo LT-Q, linear ion trap, ESI • 3x ABI Qstar (2x ESI, 1x MALDI), 2D Nanoflow HPLC • ABI Qtrap, linear ion trap, capillary LC • LC Packings Probot MALDI Spotter w/ Michrome LC • Bruker MALDI-TOF up to 350 kD • Kratos high resolution GC-MS (w/ direct inlet tube) • Varian GC-MS (ion trap w/ EI and CI)

22. Proteomics Analysis Core Services & Instrumentation: • 2D gel electrophoresis • DIGE with Typhoon Scanner (red & green laser) • High-throughput spot picking (Genomics Solution, ProPic 2) • Automated protein digestion (Genomics Solution, ProPrep 2) • 2D HPLC analysis (ProteoSep) • Isotope Coded Affinity Tagging (ICAT) • All units work in conjunction with mass spec analysis in the Center for Mass Spectrometry in the Life Sciences

23. Summary • BTI- Leading life science researchers • BRC- Leading Biotechnology services • Resources • Teaching • Industry interaction • Please come visit