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Immunoglobulin V regions diversify by gene conversion in DT40 cells

 V  V  V  V V J C 1.  V.  V  V  V  V V * J C 1. V. V *. Immunoglobulin V regions diversify by gene conversion in DT40 cells. Able to assay of sIgM+ status by flow cytometry.

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Immunoglobulin V regions diversify by gene conversion in DT40 cells

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  1. V V V V V J C1 V V V V V V* J C1 V V* Immunoglobulin V regions diversify by gene conversion in DT40 cells Able to assay of sIgM+ status by flow cytometry

  2. Chromatin features of pseudogene donors Histone acetylation H3.3 variant

  3. 14 HIRA-LacI GFP-LacI 10000 10000 12 1000 10 1000 sIGM sIGM 8 Median sIgM loss 100 100 6 10 10 4 0 0 2 0 200 400 600 800 1000 200 0 400 600 800 1000 Forward scatter Forward scatter 0 GFP-LacI HIRA Cl18 HIRA-LacI VP16-LacI 1.7 11.0 8.4 1.0 Effects on diversification

  4. HIRA-LacI LacI-HP1 GFP-LacI 71 VP16-LacI 31 36 137

  5. LacI-HP1 HIRA-LacI VP16-LacI Insertion GFP-LacI Insertion 2% 0% Point Mutation Deletion Deletion Point Mutation 11% 0% 0% 20% Short tract GC Short tract GC 3% 137 71 21% Long tract GC 66% Long tract GC 77% Long tract GC Insertion Deletion 3% 13% Short tract GC 14% 17% Deletion 0% Insertion Point Mutation 8% 13% Long-tract GC 36 45% 31 Short-tract GC Point Mutation 29% 58%

  6. HIRA-LacI-19 GFP-LacI-11 Cell 1 Cell 2 nucleosome deposition Cell 1 Cell 2 ~150 bp Effect of HIRA on chromatin MNase

  7. GFP-LacI VP16-LacI GFP-LacI-11 VP16-LacI-10 AcH4 IgG AcH4 IgG Ova V17 Enrichment: 4.5 12.4 Effect of VP16 on chromatin

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