Chapter 1—Main Themes in Microbiology

1. Chapter 1—Main Themes in Microbiology Material on Midterm Exam

2. Chapter 1 Outline • Safety & Dirty Spot lab • Dirty Spot Results—scope of Micro • 1.2-1.4 Discussion & Summary • Handwashing Lab • Graphing Quiz-size/scope-results • Scientific Method • Aseptic Technique & Pasteur ideas • Biogenesis Lab setup • Ubiquitous lab setup • Review • Test • Ubiquitous results

3. The Range and Scope of Microorganisms Microorganisms

4. PROCESS BOX 1-1 • Based on the information on the previous slide, work with your table and come up with a formal definition of microbiology MUST BE AT LEAST 3 LINES

5. Formal Definition • Microbiology is a special area of biology that deals with tiny life forms not readily observed without magnification

6. General Characteristics of Microorganisms • Organization • Prokaryotes v. eukaryotes • Size • 10nm-3mm • Lifestyles • Parasites v. free-living • All living? • Viruses

7. PROCESS BOX 1-2 • Even though we will be studying viruses in this course, why won’t we be able to “see” them? MUST BE AT LEAST 2 LINES

8. Aseptic Technique • Wash hands! (20s lather, 20s rinse) • Wipe down lab bench • Conduct lab • Flame all glass containers when opened and before closed • Flame loops before and after transfer (but let cool!) • Wipe down lab bench • Wash hands! (20s lather, 20s rinse)

9. Cornell Notes • Method of note taking • You will do for Sections 1.2-1.4 in text

10. With groups, talk about HW and agree on 3-5 key points per section • You will then be assigned ONE OF THE THREE sections

11. Student 1 Student 2 Section ______ Summary Student 3 Student 4

12. The Fall of Superstition……and the Rise of Microbiology • Spontaneous Generation • Biogenesis • Abiogenesis • Francesco Redi • Louis Jablot • Franz Shultze/Theodore Schwann • Louis Pasteur

13. START END

14. PROCESS BOX 1-3 • Why do you think Jablot and Pasteur’s closed infusions turned out to be sterile, unlike those of several other experiments? MUST BE AT LEAST 2 LINES

15. How will you know?

16. The Scientific Method

17. Scientific Method • Key terms • Independent variable • Controlled variable • Control • Dependent variable • Hypothesis • Theory • Law

18. How can I determine if bacteria is not pathogenic? Design it!

19. Antonie van Leeuwenhoek • Contribution: the microscope • Called father of bacteriology, protozoology

20. “In the said matter there were many very little animalcules, very prettily a-moving…Moreover, the other animalcules were in such enormous umbers, that all the water…seemed to be alive”

21. John Tyndall & Ferdinand Cohn • Tyndall • Some microbes have high heat resistance • Cohn • Discovered Endospores (heat-resistant structures) Concept of sterility—completely free of ALL forms of life

22. Aseptic Technique: process of handling microbes in a way that prevents infection of handler and others exposed Robert Koch Joseph Lister Aseptic Technique Oliver HolmesIgnazSemmelweis

23. Robert Koch • Koch’s Postulates • Proofs that verified germ theory • Could establish if an organism was pathogenic and which disease it caused • Bacillus anthracis THIS is how you test for pathogenicity!

25. Classification • Orderly arrangement of organisms that indicate evolutionary relationships and history • DKPGOFGS • Based on • Morphology • Structure • Physiology • Function • Genetics • Inheritance

28. What’s in a name? KNOWING LATIN AND GREEK WORD PARTS IS HUGE!

29. PROCESS BOX 1-3 • Using knowledge of Latin/Greek prefixes, predict some information about Helicobacter pylori and Thermusaquaticus. MUST BE AT LEAST 3 LINES