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MDA-MB231. HS578T. HBL-100. BEZ235, nM. C. 100. 250. C. 100. 250. C. 100. 250. pErk1/2. Erk1/2. pAkt. pS6. GAPDH.
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MDA-MB231 HS578T HBL-100 BEZ235, nM C 100 250 C 100 250 C 100 250 pErk1/2 Erk1/2 pAkt pS6 GAPDH Supplementary Figure 3. Effect of BEZ235 on the phosphorylation status of Erk1/2 in three TNBC cell lines. Cells were treated with the indicated concentrations of BEZ235 for 24 hours, and cell extracts prepared. The amounts of dually pErk1/2, Erk1/2, pAkt, pS6 and GAPDH were analysed by Western blotting with the respective antibodies. Supplementary Figure 3