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DNA Damage Response Analysis in Atm and Wip1 Genetic Models

Study evaluating p53, γH2AX, p-Brca1, cleaved PARP1, cleaved caspase-3 responses to IR in mice models. Provides insights into DNA damage signaling pathways.

sebastien-romano
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DNA Damage Response Analysis in Atm and Wip1 Genetic Models

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  1. Darlington_Supplemental Figure 1 Atm-/-Wip1+/+ Atm-/-Wip1-/- Atm+/+Wip1+/+ Atm+/+Wip1-/- p-p53 (p18S) p53 γH2AX (p140S) H2AX tubulin 5 Gy IR - - - - - - - + + + - + + + + +

  2. Darlington_Supplemental Figure 2 Atm-/- Wip1-/- Atm+/+ Wip1-/- Atm-/- Wip1+/+ Atm+/+ Wip1+/+ - - - - - - - - + + + + + + + + 5 Gy IR p-Brca1 (p1423S) Brca1 tubulin

  3. Darlington_Supplemental Figure 3 A Atm-/- Wip1-/- Atm+/+ Wip1-/- Atm-/- Wip1+/+ Atm+/+ Wip1+/+ - - - - + + - - - - + + + + + + 5 Gy IR cleaved PARP1 cleaved caspase-3 tubulin B C Cleaved PARP1 protein levels (normalized to tubulin) Cleaved Caspase-3 protein levels (normalized to tubulin) 100 100 80 80 60 60 Arbitrary unit Arbitrary unit 40 40 20 20 0 0 + + - + - + - - + + + + - - - - 5 Gy IR 5 Gy IR Atm-/- Wip1+/+ Atm+/+ Wip1-/- Atm-/- Wip1+/+ Atm+/+ Wip1+/+ Atm-/- Wip1-/- Atm+/+ Wip1-/- Atm-/- Wip1-/- Atm+/+ Wip1+/+

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