The Effect of Resveratrol on Fat Storage in C. elegans

1. The Effect of Resveratrol on Fat Storage in C. elegans By Chris LoBello

2. Obesity http://www.cdc.gov/nccdphp/dnpa/obesity/trend/maps

3. Adipocytes http://whyfiles.org/276metabolic_syndrome/index.php?g=3.txt

4. http://www.nature.com/nri/journal/v6/n10/images/nri1937-f1.jpghttp://www.nature.com/nri/journal/v6/n10/images/nri1937-f1.jpg

5. PPARg http://www.juliantrubin.com/encyclopedia/biochemistry/ppar.html

6. Chung and Kurtev (2004) http://eresveratrol.com/content/00/01/43/84/95/userimages/ppar_gamma.pdf

7. Wood and Sinclair (2004) Fei and Liu (2004) http://www.nature.com/nature/journal/v430/n7000/fig_tab/nature02789_F2.html http://www.pubmedcentral.nih.gov/picrender.fcgi?artid=1224044&blobtype=pdf

8. Control Group: 0 microliters of resveratrol N= N2 Wild Type Strain Culture Group 1: 1 microliter of resveratrol N= N2 Wild Type Strain Culture Group 2: 10 microliters of resveratrol N= N2 Wild Type Strain Culture Group 3: 50 microliters of resveratrol N= N2 Wild Type Strain Culture Group 4: 100 microliters of resveratrol N= N2 Wild Type Strain Culture Nile Red staining solution (.1microgram/ml) Done up to days 6 to 8 in the protocol Nile Red staining solution (.1microgram/ml) Done up to days 6 to 8 in the protocol RT-PCR to determine if SIRT1 is activated Quantum yield of fluorescent intensity determined using SensL photon counter (counts per nanosecond) Statistical Analysis by ANOVA where p <.05 followed by Scheffe post hoc test Methodology The Effect of Resveratrol on Fat Storage in C. elegans

9. Budget

10. Purpose To determine the effects of varying amounts of resveratrol on the fat content of C. elegans

11. Hypothesis Null Hypothesis: There will be equal amounts of fat content in cells with resveratrol supplemented and without resveratrol supplemented Alternate Hypothesis: There will be less fat content in the cells supplemented with resveratrol, and that there will be an inverse relationship between resveratrol supplementation and fat content

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