300 likes | 569 Views
Lyophilized Beer: A Banquet of Bioactive Compounds for Human Health. Jairam Vanamala, Ph.D. Assistant Professor Dept. of Food Science and Human Nutrition Colorado State University. Ancient Wisdom – Beer & Health. Mouthwash Treatment of wounds Enema Sanitation.
E N D
Lyophilized Beer: A Banquet of Bioactive Compounds for Human Health Jairam Vanamala, Ph.D. Assistant Professor Dept. of Food Science and Human Nutrition Colorado State University
Ancient Wisdom – Beer & Health • Mouthwash • Treatment of wounds • Enema • Sanitation http://philly.thedrinknation.com/articles/read/5586-Ancient-Egyptians-First-Medicinal-Use-of-Beer-#
What is in the Beer • Ethanol • Bioactive compounds • Malt – derived • Hop - derived
Beer - Protection Against Mortality • 10.14 oz. or 0.3 L/day - reduces mortality for all causes of death • Moderate beer consumption - Beneficial European Journal of Cancer41(13), 2005
Beer – Bioactive Compounds • Malt-derived compounds • Benzoic acid derivatives • Cinnamic acid derivatives • Catechins • Proanthocyanidins • Flavones
Beer – Bioactive Compounds • Hop-derived compounds • Alpha acids • Essential oils • Prenylflavonoids • Xanthohumol • Isoxanthohumol • 8-prenylnarigenin
Xanthohumol and Isoxanthohumol (I) Xanthohumol and (II) Isoxanthohumol
Is There a Synergy Xanthohumol + Hop- and malt-derived bioactive compounds
How We Test it • Colon cancer cells: HCT - 116 • Proliferation • Apoptosis
Why Colon Cancer • World: 1,000,000 • US: 1,50,000 • 80% Advanced • 2 yr survival • 80% Diet Related
Apoptosis * * * *
Xanthohumol & Isoxanthohumol – UPLC/MS/MS Xanthohumol Isoxanthohumol 2 4 6 8 10 12 14 16
Xanthohumol Content - IPAs a a b c d d d d
Critical Steps to Increase Beneficial Compounds in Beer • XAN Technology • Process • Ingredients • XN-Enriched Hop Products • Roasted Malts Xan Technology Summary Mol Nutr Food Res 49(9): 874-881
Addition of Roasted Malts - Xanthohumol a 25 % 8.7% Darker Malts b 9.94
Xanthohumol - Dry Hopping a 66 % b
Xanthohumol - DE filtration 8.7% Darker Malts .5% a a 11.1% b c 34.3% d e
Correlation Between Apoptosis & XN R2 =0.97 P = 0.0001
Future Opportunities • Lyophilized Beer • Breads • Soups • Ice creams • Protein Bars • Milk Shakes
Acknowledgements • Twila Henley • Lavanya Reddivari • Jack Avens • Marissa Bunning • Martha Stone • Jeff Miller • Breweries • Odell • New Belgium • Equinox • CopperSmith
Future Direction • Dry hopping: improve sensory characteristics and bioactive content. • Lyophilized beers • Xan Wellness & Xan Tea • Future development of XN-enriched productscould utilize the dry hopping technique.
IPAs - Antioxidant Capacity a a a ab abc abc abcd bcd cd d
IPAs - Phenolic Compounds • Compared to Pilsners • IPAs contain: • more hops • > variation in malts a ab ab bc c cd cd Roasted malts could lessen removal of phenolic compounds d d e