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Glial cells in Drosophila

Glial cells in Drosophila. 18.03.2011 Bahar. Drosophila genome. Celniker et al. 2003. Cytogenetic nomenclature. X : 1 - 20 2L : 21 - 40 2R : 41 - 60 3L : 61 - 80 3R : 81 - 100 4 : 101 - 102. Recombination. Meiotic recombination in oogenesis

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Glial cells in Drosophila

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  1. Glial cells in Drosophila 18.03.2011 Bahar

  2. Drosophila genome Celniker et al. 2003

  3. Cytogenetic nomenclature X : 1 - 20 2L : 21 - 40 2R : 41 - 60 3L : 61 - 80 3R : 81 - 100 4 : 101 - 102

  4. Recombination Meiotic recombination in oogenesis Bigger chance of recombination if the elements are further apart Shuffled balancer chromosomes to prevent recombination http://kyotofly.kit.jp/stocks/documents/Balancers.html ONLY IN FEMALES!

  5. Genetic representation Ak/Y ; UAS Gal80ts, D::E/ Sco; F gal4, UAS RFP/ TM6b, Tb X Ak/ FM7; ∆B; P{EP}C/ TM3, Act GFP, Ser http://flybase.org/static_pages/docs/nomenclature/nomenclature3.html

  6. Glia Non neural cells in CNS and PNS with ectodermic or hematopoietic origin, with the homeostasis function Glia: Neuron 1:1 Drosophila 10:1 Mammals Drosophila development atlas by Volker Hartenstein http://www.sdbonline.org/fly/atlas/00atlas.htm

  7. Glial cells Ransaohoff, 2010

  8. Embryonic development Knoblich 2001 • CNS: Lateral and midline glia. • Neurons arise from neuroblasts and gmc. • Glia arise from glioblast and neuroglioblast Lateral glia development initiated by gcm. (transcription factor) repo downstream of gcm, another transcription factor

  9. Embryonic development Bashaw 2010 Axons Neurons Banerjee 2007 Commissures and bundles

  10. Larval glia Zuo 2008 NMJs Brain Imaginal discs Aigouy 2004 Soustelle 2007

  11. Bristles Neumüller 2009

  12. Useful drivers Repo gal4 (aka M1B): pan glial driver. CNS and PNS entire glia, starting from early embryonic development, continues after terminal differentiation. Larval and adult glia. Gcm gal4: embryonic glial driver. neuroglioblast/glioblast-specific driver, limited to development. Restricted expression in larva.

  13. Glial drivers Mz97gal4: embryonic and larval glia (Read, 2009). Peripheral and wrapping glia. But it’s not restricted to glia. Nrv2gal4: embryonic to adult CNS glial (Read, 2009) Eaatgal4: larval cortex glia in CNS, perisynaptic glia in PNS (Read, 2009)

  14. Glial drivers Moody gal4, surface glia in CNS (Lievens, 2008) Rhomboid gal4, embryonic mesectodermal/midline glial driver Gliotactin gal4, peripheral glia, for NMJs (Lavery, 2007) Gal4-NP577, cortex glia (PNS) (Kato, 2008)

  15. Glial drivers MZ709, embryonic and first star larval, peripheral glia (Lavery, 2007) 158 gal4, lateral glial driver New tools for glia http://onlinelibrary.wiley.com/doi/10.1002/glia.21133/full

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