1 / 6

Tests for biological macromolecules

Tests for biological macromolecules. Benedict’s Solution. Test for simple (reducing) sugars Benedict’s is blue Put test substance in a test tube with distilled water and a few drops of B enedict’s Heat gently in a water bath for two minutes

solada
Download Presentation

Tests for biological macromolecules

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript

  1. Tests for biological macromolecules

  2. Benedict’s Solution Test for simple (reducing) sugars Benedict’s is blue Put test substance in a test tube with distilled water and a few drops of Benedict’s Heat gently in a water bath for two minutes It should change color from blue to green to yellow/orange/red There could be an orange precipitate

  3. Iodine test Tests for starch Iodine is orange Iodine can be put directly on a test substance or into a solution Positive test is orange turns to black Instantaneous

  4. Biuret solution Solution of NaOH and CuSO4 Test for proteins Put substance in a test tube and add Biuret Color change from blue to lavender is positive No heat but not instantaneous

  5. Ethanol Test for fats and oils Put substance in a container (test tube) and add ethanol (shake/crush) Heat in a gentle water bath (not Bunsen Burner!!!) Filter or dilute (with more ethanol) to get a clear liquid Pour into a test tube of water A white (milk-like) emulsion indicates presence of fats/oils

  6. Sudan Red Test for lipids Place 1 mL of sample in 1 mL of water and mix Add 5 drops of Sudan Red and mix Wait 2 minutes Lipid is present in red-stained layer

More Related