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Use of Fluorescence in the study of DNA

Use of Fluorescence in the study of DNA. Presented By: Jeff Abey Mishal Asim Jayani Jayasuriya Noor Khan. What is Fluorescence?. Emission of light by a substance that has already absorbed light or other electromagnetic radiation.

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Use of Fluorescence in the study of DNA

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  1. Use of Fluorescence in the study of DNA Presented By: Jeff Abey Mishal Asim Jayani Jayasuriya Noor Khan

  2. What is Fluorescence? Emission of light by a substance that has already absorbed light or other electromagnetic radiation

  3. What is Fluorescence Microscopy? • Technique using a light microscope to study properties of organic or inorganic substances • Used in studying DNA, its metabolism and structure

  4. In Situ Hybridization • In Situ means: in natural positions within a chromosome • Hybridization: The process of forming a double standard nucleic acid from joining two complementary strands of DNA (or RNA)

  5. FISH • “Fluorescence in Situ Hybridization” • Method used to determine the chromosomal location or expression pattern of genomic DNA • Versatile procedure

  6. Enzymes and FISH • Used due to their simplicity, sensitivity and broad- spectrum applications • Enhanced detection of DNA

  7. Applications of FISH • Determine where children’s disabilities come from • Identify different species

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