FZD7 Supplementary figures

1. FZD7 Supplementary figures

2. Supplementary Figure 1 A) SKOV3-P 2D Parental SKOV3-S 3D Spheroid SKOV3-S2D Spheroid-reattached B)

3. Supplementary Figure 2 FZD7-6 siRNA FZD7-5 siRNA NEG. CONTROL A) CH1 PA-1 OV-17R PI Annexin-V

4. Supplementary Figure 2 ANNEXIN V B) C) D)

5. Supplementary Figure 3 B) A) C) D)

6. Supplementary Figure 4 b-cat F-actin Merge CH1 b-cat F-actin Merge PA-1 b-cat F-actin Merge OV-17R NEG. CONTROL FZD7-6 FZD7-5

7. Supplementary Figure 5 CH1 PA-1 OV-17R NEG. CONTROL FZD7-6 FZD7-5

8. Supplementary Figure 6 CDC42

9. Supplementary Figure 7 A) CH1 0-hrs 15-hrs FZD7-5 siRNA FZD7-6 siRNA NEG. CONTROL

10. Supplementary Figure B) PA-1 0-hrs 15-hrs FZD7-5 siRNA FZD7-6 siRNA NEG. CONTROL

11. Supplementary Figure C) OV-17R 0-hrs 32-hrs FZD7-5 siRNA FZD7-6 siRNA NEG. CONTROL

12. Supplementary Figure 8 A) CH1 Merge γ-tubulin Ck1ε Merge γ-tubulin DVL2 pSer143 NEG. CONTROL Merge γ-tubulin Ck1ε Merge γ-tubulin DVL2 pSer143 FZD7-5 Merge γ-tubulin Ck1ε Merge γ-tubulin DVL2 pSer143 FZD7-6

13. Supplementary Figure 8 B) PA-1 Merge γ-tubulin DVL2 pSer143 Merge γ-tubulin Ck1ε NEG. CONTROL γ-tubulin DVL2 pSer143 Merge γ-tubulin Ck1ε Merge FZD7-5 Merge γ-tubulin DVL2 pSer143 Merge γ-tubulin Ck1ε FZD7-6

14. Supplementary Figure 8 C) OV-17R Merge γ-tubulin Ck1ε Merge γ-tubulin DVL2 pSer143 NEG. CONTROL Merge γ-tubulin Ck1ε Merge γ-tubulin DVL2 pSer143 FZD7-5 Merge γ-tubulin Ck1ε Merge γ-tubulin DVL2 pSer143 FZD7-6

15. Supplementary Figure 9 FZD7-5 siRNA FZD7-6 siRNA FZD7-6 siRNA FZD7-5 siRNA +Wnt5a +Wnt5a +Wnt5a +Wnt5a B)PA1 A)CH1 -Wnt5a -Wnt5a -Wnt5a -Wnt5a +Wnt5a +Wnt5a C)OV-17R -Wnt5a -Wnt5a