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Genetic Technology

Genetic Technology. Using molecular genetics for practical purposes. Must first identify the genome of the organism= all genes within the species Human Genome Project: 1990’s- Thought there were 100,000 genes. Only found 30,000. Pieces of desired plant placed into growth medium

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Genetic Technology

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  1. Genetic Technology Using molecular genetics for practical purposes

  2. Must first identify the genome of the organism= all genes within the species • Human Genome Project: 1990’s- Thought there were 100,000 genes. Only found 30,000.

  3. Pieces of desired plant placed into growth medium -Food Supply (Salmon, fruits, veggies, corn) • Somatic cell nuclear transfer Cloning

  4. Undifferentiated cells (early development) can “switch” on DNA and “tell” cell what to be. • May be a cure for Parkinson’s, “growing” organs, tissues for burn treatment, paralysis • Controversial • 2types: adult and embryonic Stem Cell

  5. Treat genetic disorder by replacing “bad” gene with a “good” one. • Designing medications specific to a person’s genetic code • Could cure: Hemophilia, Cystic Fibrosis, Muscular Dystrophy Gene Therapy

  6. Using genetic code markers to identify people • Used for paternity tests and test DNA found at crime scenes. • Gel electrophoresis- Bar code of genetic markers. DNA Profiling (fingerprinting)

  7. In past genetic tests on small samples used up most or all of the DNA • Makes many copies of small amounts of DNA for testing. PCR: Polymerase Chain Reaction

  8. 1970: Bacteria E. coli makes ENDONUCLEASES (enzymes) Cuts viral DNA in the same place EVERYTIME. “Sticky Ends” Results are: smaller pieces of DNA, some the same size GAATTC CTTAAG

  9. Paternity Forensics

  10. Most promising for curing disorders. • Making human proteins using bacteria: - Insulin: treatment of diabetes - Clotting Factor: hemophilia - HGH: height disorders • Combines genes that code for human proteins with the plasmid DNA of E.coli…which now synthesizes the human protein Recombinant DNA

  11. SPORTS CAR

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