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Supplementary data. (C). (A). --. +. KO KO KO HE HE HE. P5 P6 P7 P8. Right arm of long PCR. 5’. WT allele. Left arm of long PCR. Exon 1 Exon2. Left arm of wt. P9 P10 P11 P12. 1 Kb. 5’. Left arm of ko. HdIII. HpaI. BamHI. SalI. Targeted allele.
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Supplementary data (C) (A) -- + KO KO KO HE HE HE P5 P6 P7 P8 Right arm of long PCR 5’ WT allele Left arm of long PCR Exon 1 Exon2 Left arm of wt P9 P10 P11 P12 1 Kb 5’ Left arm of ko HdIII HpaI BamHI SalI Targeted allele Right arm of wt P1 P2 P3 P4 Right arm of ko (B) (D) WT KO KO WT -- P T11 -- D36 -- JCN Left arm 3.25KB CSQ Right arm 2.25KB
