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Purdue iGEM 2012

Purdue iGEM 2012. Meeting Friday 6/15/12. Recap of the Past Week’s Work. Trouble shooting with transformations Discovery of bad sequencing in AIDA-1 Primer construction Order Gibson Plasmids Meeting with Janie and Dr. Litster Research on experiments to characterize constructs.

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Purdue iGEM 2012

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  1. Purdue iGEM 2012 Meeting Friday 6/15/12

  2. Recap of the Past Week’s Work • Trouble shooting with transformations • Discovery of bad sequencing in AIDA-1 • Primer construction • Order Gibson Plasmids • Meeting with Janie and Dr. Litster • Research on experiments to characterize constructs

  3. Things to Discuss this Week • Curli, Ag43, AIDA-1, FimA-H • Transformation failures • Primer designs for Gibson Assembly • Organization of growing group • Committees? • Use of Wiki

  4. Plan for the Next Week • Trouble Shoot with transformations • Continue transformations • Set up controls and experimental plans to get data to optimize setup using JMP • Read another paper?

  5. Journal Review! • Everyone read a paper on either syn bio or an area related to the project • Wrote short summary to be uploaded to drop box • Present that info now 

  6. Committees • Experimental Design – Statistics/make product work • Mrudula* • Chris • Arthi • Modeling - JMP • Max* • Haefa • Wiki (Notebook/Admin) • Rachel* - collaborating • Background – Sean • Committees update as well • Characterizing – parts specific • Amanda* • Chris • Rachel • Wetlab Work • OmpA-Sil • August* • Haefa • AIDA-1 • Rubeena* • Purchasing • Peter* – cc him on all e-mails so he can keep a list • Human Practices • Peter* • Max* • Project Planning • Namita

  7. To Do Next Week • Wetlab to do (Rubeena/August): • Transformation trouble shooting - if still not working call iGEM to get new cells • Check the concentration of the DNA in the wells with nanodrop (should get 20) • Contamination with cells we have? • Re-competent the cells from Janie • Check the concentration of Amp • Get one transformation to work before continuing – check with Soo’s protocol from workshop • Call iGEM TODAY!!!!!!!!! • Grow biofilms! • Ask Eric McLamore about how best to make biofilm/bioreactor • Detatchment assay • Make Primers for Curli for the biobrick plasmid • Rachel – give to Wetlab committee (Rubeena) • Purchase the primers • Read a paper - everyone • Start rotating describing the project to the group – set up rotation – Amanda for Friday August next Friday • Order OmpA-Silicatein construction to be synthesized! • Bring forms to Tuesday meeting for purchasing • Committees meet and set up To-Do lists and meetings within committee

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