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Scientific Talks in general and

Scientific Talks in general and. Masterpiece Theatre in particular. The Ten Minute Rule. Step 1 : Isolate the Take Home Message (THM). Distill your data down to one point Then state the significance in one sentence. Fall 2004 Speakers. Examining mechanisms of cell division

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Scientific Talks in general and

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  1. Scientific Talks in general and Masterpiece Theatre in particular.

  2. The Ten Minute Rule

  3. Step 1 : Isolate the Take Home Message (THM) Distill your data down to one point Then state the significance in one sentence

  4. Fall 2004 Speakers Examining mechanisms of cell division using real-time microscopy and chemical genetics Tarun Kapoor Genome restoration after radiation damage in Deinococcus radiodurans Mike Cox

  5. Step 2 The Introduction Purpose- Start from nothing and get to the THM Remember: Who’s your Daddy? Just the Facts

  6. Determine the “main question” or hypothesis • Very Specific • The answer will have broad implications and be the THM Remember: Who’s your Daddy? Just the Facts

  7. Introduction (The question) Results (The data and interpretation) Conclusion (The THM )

  8. Q. How much data? Q. How many slides? A. = T/2 (rule of thumb)

  9. The main question dictates the data you present YES Data or Slide or Overhead ? NO Who’s your Daddy?

  10. Results Section • Longest section = .7 T • Logical flow • Avoid vague statements • Draw conclusions (different from THM) Conclusion: Mutations in abc1 cause abnormal eye development THM: Eye development is regulated by ubiquitylation

  11. Know Your Data • Practice without it • What the data are • What the data mean • Where the data lead

  12. Step 3 The Conclusion • Recap the question • Recap the most important conclusion(s) • State the THM

  13. The Three Tellums 1. Tellum what you’re gonna tellum 2. Then tellum 3. Then tellum what you toldum

  14. Slides - audiovisual aids

  15. Tor signaling • Tor is a highly conserved PI3 (lipid) kinase that is a protein kinase • in vitro, and presumably in vivo. • So-called because it is the “target of rapamycin” • Rapamycin is a drug that was identified from the soil of the • island of “Rapa Nui” (also known as Easter Island) in a screen for • anti-fungal compounds • Yeast mutants resistant to rapamycin identified three genes • TOR1TOR2 and FRP1 • FRP1 encodes a homolog of FK-506 binding protein • It is rapamycin bound to FK-506 binding protein that binds to and • inhibits the Tor1 and Tor2 protein kinases

  16. WCE Immunodeplete Mad3-PrA (IgG Sepharose) Mad3-PrA (IgG IP) SUP (IgG IP) Mad2 Immunodeplete Cdc20-13Myc (anti-Myc beads) (Myc IP) SUP (Myc IP) A C WCEIgG IPSup-IgG IP Mad3-PrA - + + - + + - + + Cdc20-13Myc + - + + - + + - + YEPD + Ben YEPD 1 2 3 4 5 6 7 8 9 Wild type MAD3-PrA MAD3-PrA CDC20-13MYC Dmad2 B D Sup-IgGIPIgG IPMyc IPSup- Myc IP Mad3-PrA - + + - + + - + + - + Cdc20-13Myc + - + + - + + - + + + 1 2 3 4 5 6 7 8 9 10 11 Cdc20-13Myc Mad2

  17. (Myc IP) SUP (Myc IP) WCE SUP (IgG IP) Immunodeplete Mad3-PrA (IgG Sepharose) Immunodeplete Cdc20-13Myc (anti-Myc beads) (IgG IP)

  18. (Myc IP) SUP (Myc IP) WCE SUP (IgG IP) Immunodeplete Mad3-PrA (IgG Sepharose) Immunodeplete Cdc20-13Myc (anti-Myc beads) (IgG IP)

  19. (Myc IP) SUP (Myc IP) WCE SUP (IgG IP) Immunodeplete Mad3-PrA (IgG Sepharose) Immunodeplete Cdc20-13Myc (anti-Myc beads) (IgG IP)

  20. (Myc IP) SUP (Myc IP) WCE SUP (IgG IP) Immunodeplete Mad3-PrA (IgG Sepharose) Immunodeplete Cdc20-13Myc (anti-Myc beads) (IgG IP)

  21. WCE SUP (IgG IP) Immunodeplete Mad3-PrA (IgG Sepharose) Immunodeplete Cdc20-13Myc (anti-Myc beads) (IgG IP) (Myc IP) SUP (Myc IP)

  22. WCE Immunodeplete Mad3-PrA (IgG Sepharose) Mad3-PrA (IgG IP) SUP (IgG IP) Mad2 Immunodeplete Cdc20-13Myc (anti-Myc beads) (Myc IP) SUP (Myc IP) A C WCEIgG IPSup-IgG IP Mad3-PrA - + + - + + - + + Cdc20-13Myc + - + + - + + - + YEPD + Ben YEPD 1 2 3 4 5 6 7 8 9 Wild type MAD3-PrA MAD3-PrA CDC20-13MYC Dmad2 B D Sup-IgGIPIgG IPMyc IPSup- Myc IP Mad3-PrA - + + - + + - + + - + Cdc20-13Myc + - + + - + + - + + + 1 2 3 4 5 6 7 8 9 10 11 Cdc20-13Myc Mad2

  23. (Myc IP) SUP (Myc IP) WCE SUP (IgG IP) Immunodeplete Mad3-PrA (IgG Sepharose) Immunodeplete Cdc20-13Myc (anti-Myc beads) (IgG IP)

  24. Sequential Immunoprecipitation WCE PrA Myc Mad3 Cdc20 SUP Pull down Mad3-PrA Mad2 Mad2 (Blot Mad2) Pulldown Cdc20-13Myc (Blot Mad2)

  25. Sup-IgGIPIgG IPMyc IPSup- Myc IP Mad3-PrA - + + - + + - + + - + Cdc20-13Myc + - + + - + + - + + + 1 2 3 4 5 6 7 8 9 10 11 Cdc20-13Myc Mad2

  26. Sup-IgGIPIgG IPMyc IPSup- Myc IP Mad3-PrA - + + - + + - + + - + Cdc20-13Myc + - + + - + + - + + + 1 2 3 4 5 6 7 8 9 10 11 Cdc20-13Myc Mad2 PrA Myc Mad3 Cdc20 Mad2 Mad2

  27. PrA Myc Mad3 Cdc20 Mad2 Mad2 Sup-IgGIPIgG IPMyc IPSup- Myc IP Mad3-PrA - + + - + + - + + - + Cdc20-13Myc + - + + - + + - + + + 1 2 3 4 5 6 7 8 9 10 11 Cdc20-13Myc Mad2

  28. PrA Myc Mad3 Cdc20 Mad2 Mad2 Sup-IgGIPIgG IPMyc IPSup- Myc IP Mad3-PrA - + + - + + - + + - + Cdc20-13Myc + - + + - + + - + + + 1 2 3 4 5 6 7 8 9 10 11 Cdc20-13Myc Mad2

  29. The Delivery “Every product of genius is a product of enthusiasm” Benjamin Disreali

  30. And finally………….. Dress for Success

  31. No matter what the talk/seminar is: The Ten Minute Rule T/2 slides Be professional

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