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DMD #031831

DMD #031831 A comparison of whole genome gene expression profiles of HepaRG cells to human liver tissues, primary human hepatocytes, and HepG2 cells Steven N. Hart*, Ye Li*, Kaori Nakamoto, Eva-anne Subileau, David Steen, and Xiao-bo Zhong

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DMD #031831

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  1. DMD #031831 A comparison of whole genome gene expression profiles of HepaRG cells to human liver tissues, primary human hepatocytes, and HepG2 cells Steven N. Hart*, Ye Li*, Kaori Nakamoto, Eva-anne Subileau, David Steen, and Xiao-bo Zhong Supplemental Figure 1S. A comparisonof the expression values in Log2 scale among HepG2 cells, differentiated HepaRG cells, and primary human hepatocytes for CYPs in panel A, ADH in panel B, ALDHs in panel C, Non-CYP phase I enzymes in panel D, GSTs in panel E, NATs in panel F, SULTs in panel G, UGTs in panel H, ABCB/ABCC/ABCG in panel I, and SLCOs in panel J. When multiple sets of probes related to a gene are presented on the arrays, the probe that is most variable for a given gene is selected to represent the gene. The bars show the average expression values in Log2 scale and the error bars indicate standard deviation. **p<0.01 and ***p<0.001 are used to indicate a statistically significant difference compared to primary human hepatocytes in a t test. UGT1Aa corresponds to multiple UGT1A family members, including UGT1A1, UGT1A3, UGT1A4, UGT1A8, UGT1A9, and UGT1A10.

  2. Supplemental Figure S1. A. CYPs HepG2 Diff HepaRG PHH ** ** ** Gene expression (Log2) ** *** *** *** *** *** ** *** *** *** *** *** *** *** B. ADHs ** ** ** ** Gene expression (Log2) ** *** *** *** **

  3. Supplemental Figure S1. C. ALDHs ** *** *** *** *** *** ** Gene expression (Log2) *** *** *** *** ** *** ** ** ** ** ** *** D. Non-CYP Phase I ** ** Gene expression (Log2) ** ** ** ** *** *** *** ***

  4. Supplemental Figure S1. E. GSTs ** *** ** *** ** ** Gene expression (Log2) ** ** ** *** ** *** ** *** ** F. NATs ** ** *** ** ** ** ** ** ** ** *** Gene expression (Log2) *** *** *** ***

  5. Supplemental Figure S1. ** ** ** ** ** *** ** ** *** *** *** *** *** *** a G. SULTs ** *** ** ** Gene expression (Log2) *** ** *** ** ** H. UGTs ** ** ** ** Gene expression (Log2) ** *** ** *** *** *** *** ***

  6. Supplemental Figure S1. I. ABCB/ABCC/ABCG ** *** ** *** *** ** ** *** *** *** ** ** ** ** Gene expression (Log2) *** ** ** ** ** ** J. SLCOs Gene expression (Log2) ** *** ***

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