1 / 2

RB GUS P Ubi TEVL Xa3 NOS Hpt LB

BamH I. BamH I. A. Clone MKbF in vector pU1301. B. BamH I. BamH I. HindIII. -691. +37. RB P WRKY13 Xa3 NOS Hpt LB. Clone 12IMKb in vector pI1381. Dra I. Dra I. C. Clone MKb in vector pCAMBIA1301.

virote
Download Presentation

RB GUS P Ubi TEVL Xa3 NOS Hpt LB

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript

  1. BamHI BamHI A Clone MKbF in vector pU1301 B BamHI BamHI HindIII -691 +37 RB PWRKY13 Xa3 NOS Hpt LB Clone 12IMKb in vector pI1381 DraI DraI C Clone MKb in vector pCAMBIA1301 RB GUSPUbi TEVL Xa3 NOS Hpt LB RB GUSPXa3 Xa3 NOS Hpt LB Supplemental Figure 1.Schematic diagrams of the transformation constructs. RB and LB, right and left T-DNA border; GUS, -glucuronidase gene; Hpt, hygromycin phosphotransferase gene; PUbi, maize ubiquitin gene promoter; TEVL, 5‘-nontranslated region of the tobacco etch virus; NOS, nopaline synthase polyadenylation signal. (A) overexpression construct of Xa3, MKbF, containing the complete genomic sequence of Xa3 coding region. (B) Pathogen-induced construct of Xa3, 12IMKb, containing the complete genomic sequence of Xa3 coding region. PWRKY13 is OsWRKY13 gene promoter locating at -691 to +37 of OsWRKY13 of rice variety Minghui 63. (C) Construct containing a 7.5-kb DNA fragment harboring Xa3 and its native promoter (PXa3) from Minghui 63.

  2. 10 Zhonghua 11 background Minghua 63 9 8 MKbZH2 (PXa3:Xa3) Zhonghua 11 (wild type) 7 MKbFZH1 (PUbi:Xa3) 6 5 4 3 2 Bacteria [log (cfu/leaf)] Mudanjiang 8 background 10 Minghua 63 9 Mudanjiang 8 (wild type) 8 Rb17-2 (PXa3:Xa3) 7 MKbFMDJ2 (PUbi:Xa3) 6 5 4 3 0 6 12 2 Days after inoculation Supplemental Figure 2.Growth of Xoo strain PXO61 in the leaves of rice plants at four-leaf stage. Indica rice variety Minghua 63 is the donor of Xa3. Japonica rice varieties Zhonghua 11 and Mudanjiang 8 are wild type plants. MKbZH2, MKbFZH1 and MKbFMDJ2 are resistant transgenic plants of T1 generation and Rb17-2 is homogeneous transgenic line. The bacterial population was determined from three leave at each time point by counting colony-forming units (cfu).

More Related