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DNA Fingerprinting of Garlic Accessions

Workpackage 1: Genetic Resources. DNA Fingerprinting of Garlic Accessions. Ria Vrielink Chris Kik Sjaak van Heusden. Screening for genetic variation. Method: AFLP-fingerprinting ( "fast and indicative") Standard: Maass and Klaas dendrogram Theor Appl Genet (1995) 91:89-97 Question:

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DNA Fingerprinting of Garlic Accessions

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  1. Workpackage 1: Genetic Resources DNA Fingerprinting of Garlic Accessions Ria Vrielink Chris Kik Sjaak van Heusden

  2. Screening for genetic variation Method: • AFLP-fingerprinting ( "fast and indicative") • Standard: Maass and Klaas dendrogram • Theor Appl Genet (1995) 91:89-97 Question: • How representative is our garlic collection for the whole gene pool of garlic?

  3. Group Number Origin Ia 2 Georgia/Caucasus Ic 3 Slovakia/Georgia/Caucasus IIa 2 Korea/China IIb 3 Yugoslavia/Romenia IIc 6 Slovakia/Georgia/Caucasus/Italy/Korea IId 3 Georgia/Caucasus III 6 Romenia/Georgia/Caucasus/USA/Australia IVb 3 Korea/Japan IVe 2 China/Kazakstan

  4. Amplified Fragment Length Polymorphism (AFLP) • Many markers possible • Reliable • No prior sequence knowledge necessary • Genome wide distribution

  5. Garlic groups III I and IV IIa,b; IIc,d V

  6. 97015 97076 97078 99005 99006 99007 99008 99066 99067 99068 99069 97015 97076 97078 99005 99006 99007 99008 99066 99067 99068 99069 1 1 1 0 0 0 1 0 0 0 1 1 1 1 1 1 1 0 1 1 1 1 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 1 1 1 0 1 1 1 0 1 0 1 1 1 1 1 One AFLP enzymeprimer combination Result: 20 informative differences

  7. Garlic accessions to be analyzed • From collection Gatersleben 139 accessions • for comparison with Maass and Klaas • some new accessions in garlic collection • Own G&H collection of 84 accessions • R. Kamenetsky, H.D. Rabinowitch, L. van Soest and others

  8. Outgroup All143 All649 All782 3 accesions No accesions All793 5 accesions 2 in “core” 11 accesions 2 in “core” 4 accesions 2 in “core” All804 All827 All839 All853 All936 All854 All816 All812 All808 All655 97355 97068 All822 97066 All820 97080 97073 All784 97065 All792 97360 97357 97356 97354 97353 97015 97078 97076 97081 Tax452 All908 All785 All766 97086 97085 97087 97084 97083 97089 97088 97092 97091 All129 97072 97071 97070 97069 97067 All877 97082 97075 All787 All902 All901 All845 All842 All652 1997 Garlic Accessions II c,d II a,b I a,b,c IV b IV a,b,c,d III

  9. 1997 accessions III I and IV 0 6/14 3 II a,b; II c,d V 0 Unclear: 6 accessions

  10. 1999 accessions III I and IV 1 10/3 10 II a,b; II c,d V 0 Unclear: 5 accessions

  11. 2000 accessions III I and IV 0 5/5 14 II a,b; II c,d V 0 Unclear: 2 accessions

  12. 99005 (American Early II) 99006 (Morado III), 99073, 20022 26 accessions representing the complete variation in the collection. 97356, 99069, 97073, 99078, 99081, 20007, 20027 I a,b,c 97068, 99067, 99071, 20005, 20008, 20025 II a,b II c,d III 99008 99074, 97015, 97081 IV b,c,e 97069, 97080, 97085, 97089, 97091 IV ? ??

  13. Conclusions • AFLP-fingerprinting good method to minimize the collection • duplications out • very closely related accessions out • No accessions in subtropical group and only one in ophioscrodon group

  14. Future plans • Comparison new accessions with the core collection until now • Other determinations on core collection

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