1 / 10

Adhesion-Based Cell Sorter with Antibody-immobilized Functionalized-Paryene Surface

Adhesion-Based Cell Sorter with Antibody-immobilized Functionalized-Paryene Surface. Author ： Junichi Miwa, Yuji Suzuki and Nobuhide asagi Department of Mechanical Engineering,The University of Tokyo Hongo 7-3-1,Bunkyo-ku, Tokyo 113-8656,Japan Reporter: Wun-Hao Wu ( 吳文豪 )

wynona
Download Presentation

Adhesion-Based Cell Sorter with Antibody-immobilized Functionalized-Paryene Surface

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript

  1. Adhesion-Based Cell Sorter with Antibody-immobilized Functionalized-Paryene Surface Author：Junichi Miwa, Yuji Suzuki and Nobuhide asagi Department of Mechanical Engineering,The University of Tokyo Hongo 7-3-1,Bunkyo-ku, Tokyo 113-8656,Japan Reporter:Wun-Hao Wu (吳文豪) 11/21, 2007

  2. Outline • introduce • the device work principle • fabrication • result • conclusion

  3. introduce Nature Biotech., vol.17 , no.11,pp.1109-1111.1999 BIOLOGY OF REPRODUCTION 61, 582–589 (1999) The cell sorter is more merit of label-free sorting and the potential for higher throughput .

  4. diX AM substrate dix AM μTAS ’05 ,Boston ,MA,USA ,Oct,9-13,2005,pp.868-870. • Two advantage of using diX AM for immunoreactive surface . • It is provided as-deposited on conformally-coated three dimensional structures. • It is highly biocompatible in nature.

  5. principle device μTAS ’05 ,Boston ,MA,USA ,Oct,9-13,2005,pp.868-870. • Adsorbing the Antibodies • Cell inject into the microchannel • Passing through the Sample plug • Then, Passing through the Cell sorting column.

  6. Adsorbing the Antibodies • Conjugating NHS-LC-LC-biotin to surface amines. • NHS-LC-LC-biotin is dissolved into dimethylsulfoxide. • pouring into bicine buffered saline(pH 8). • Biotin solution is introduced into micro column and incubated for one hour at 30 ℃. • Streptavidin and biotin-conjugated CD31 antibody solution is,each of them dissolved into PBS(pH 7.4).

  7. Fabrication

  8. result HUVEC Antibody Time-averaged HUVEC velocity in microcolumns with various surface antibody immobilizations.

  9. HUVEC and HL60 are mixed at 5 ×105 cells/mL total concentration, at the ratio of 1:1. The flow is driven by a syringe pump at the total flow rate of 1.2 μL/min, with the volume ratio between the main inlet and two hydeodynamic focousing buffer inlets being 2:1:1. the corresponding bulk mean flow velocity in the cell sorting column is 1 mm/s

  10. Conclusion • Label-free sorting. • Higher throughput • In the future, We can identify for some our different bodies cells, such as stem cells or monocyte. It can help us to recognize how it work.

More Related