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Constructing E.coli cells to act like multicellular organisms

Constructing E.coli cells to act like multicellular organisms. ——The engineered evolutionary story. Approaching to this purpose. One-dimension Two-dimension Three-dimension Mathematical modeling. Do E.coli cells have a head?. One-dimension organization. The simplest form----two cells.

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Constructing E.coli cells to act like multicellular organisms

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  1. Constructing E.coli cells to act like multicellular organisms ——The engineered evolutionary story

  2. Approaching to this purpose • One-dimension • Two-dimension • Three-dimension • Mathematical modeling

  3. Do E.coli cells have a head?

  4. One-dimension organization • The simplest form----two cells • What we need are: • A protein located at the anterior side of E.coli cells. • A self-recognizable domain that act both as a ligand and a receptor.

  5. The anterior located protein • Chemoreceptor Complex in Escherichia coli. Tsr CheA CheW

  6. The outer membrane domain can be modified • By the monomer of leucine zipper or the IgSF domain. • By the hexomer of histidine.

  7. Two-dimension organization • What we need are: • A protein located in the anterior site that can bind to a shaped mode. • A membrane protein that is broadly distributed on the surface of E.coli cells.

  8. OmpC protein • An outer membrane protein.

  9. Cell Division Control • SulA and MinCD • Enrolled in SOS.

  10. Three-dimension: the story of evolution • When cell density increases: • Expression of modified OmpC via quorum sensing system. (eg. Inserting constructed OmpC sequence after the promoter of LuxR) • Randomly bind?

  11. Mathematical modeling • What distinguishes multicelluar organisms from unicellular ones. • Evolutionary fitness and trade-off.

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