041805 C57E18.5 P(KO)vs P(WT)

Nov 19, 2014

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041805 C57E18.5 P(KO)vs P(WT)

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Role of cytokines and lymphocytes on the efficacy of Posaconazole and Benznidazole in treatment of experimental acute T

Role of cytokines and lymphocytes on the efficacy of Posaconazole and Benznidazole in treatment of experimental acute Trypanosoma cruzi infection Alvaro José Romanha Centro de Pesquisa René Rachou – FIOCRUZ - MG. Rio de Janeiro, July 2009. WT. IFN-  KO. IL-12 KO. WT. IFN-  KO.

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What’s the “Beef” with Soy P rotein?

What’s the “Beef” with Soy P rotein?. By: Brent Fritsch. Introduction. ACSM Recommends protein intakes of 1.2-1.7g/kg body wt. for endurance and strength-trained athletes Some strength-trained athletes consume as much as 3.0g/kg body wt.

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Supplementary Figure 1

WT. WT. WT. KO. KO. KO. Supplementary Figure 1. A. Body weight. B. Vision. C. Electroretinogram. D. Open field. Rotarod. E. p=0.34. 400. WT. WT. 17.5. 75. KO. KO. 15.0. 300. WT. 12.5. 50. KO. Body weight [g]. Preference [%]. Amplitude ( μ V ). Time [s]. 10.0.

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Similar to “up in WT tumor”

Similar to “up in WT tumor”. Anticorrelated with “up in wt tumor”. Similar to “up in KO tumor”. KO is “induced” with oil treatment (Sim to pattern 1; 61 genes). BALB KO. oil. BHT-1d. BHT-3d. oil. BHT-1d. BHT-3d. More genes to add to pattern 1; Sim to pattern 2; 110 genes. BALB KO.

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I. Selections: I-AniI for wild-type site binding/cleavage: “WT-opt” (Ryo)

I. Selections: I-AniI for wild-type site binding/cleavage: “WT-opt” (Ryo) I-AniI towards hCF (Audrey) I-AniI towards A.gam. CTLMA2 (Ryo) II. Binding specificity profiles: I-CreI, I-MsoI, I-AniI (WT vs. wt, WT vs. lib4, WT-opt vs. wt), I-SspI III. Crystal Structures:

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GNAS and Fat

GNAS and Fat. KO. WT. White Adipose Tissue. KO. WT. WT. Brown adipose tissue. Fat Pads. WT KO. BAT. BAT. WAT. WAT. Observation: small fat pads in KO Possible reasons: Fewer adipocytes but same size Same number of adipocytes, but smaller (contain less lipid). (1). (2). KO.

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P-STAT2

A. B. P117. WT. Genomic sequence. cDNA sequence (P117). IFN a 3 h 6h. IFN g 0 3 h 6h. IFN a 3 h 6h. IFN g 0 3 h 6h. P117. WT. Exon 4. Exon 5. P-STAT1. STAT1. P-STAT2. STAT2. P-STAT3. STAT3.

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3500x

Supplementary Figure 1. A. TiO 2. 3500x. 8900x. B. Carbon black. 5600x. 14 000x. C. SWCNT. 1500x. 5000x. Supplementary Figure 2. P-2 κ B α immunostaining of WT and p53 KO macrophages. A. DMEM. SWCNT. p53 KO. WT. WT. p53 KO. Immunostaining quantification. B.

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WT control

Figure S1. WT control. A3R KO control. WT-TAC. A3R KO-TAC. Cell size. Fibrosis.

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HpaI

Supplementary data. (C). (A). --. +. KO KO KO HE HE HE. P5 P6 P7 P8. Right arm of long PCR. 5’. WT allele. Left arm of long PCR. Exon 1 Exon2. Left arm of wt. P9 P10 P11 P12. 1 Kb. 5’. Left arm of ko. HdIII. HpaI. BamHI. SalI. Targeted allele.

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PCAF WT

PCAF WT. PCAF KO. A. B. E. Ctl. C. D. Obj. Recogn. F. G. J. Ctl. H. I. Obj. Recogn. K. L. O. Ctl. M. N. Obj. Recogn.

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Supplementary Figure S1, Schneider et al

Supplementary Figure S1, Schneider et al. Fully migrated out of seeded (FM ratio). A. medium. WT sup. total PBMC. NS. KO sup. medium. NS. NS. NS. NS. WT sup. lymphocytes. NS. KO sup. medium. monocytes. WT sup. NS. KO sup. 0. 10. 20. 30. 40. 50. FM ratio (%).

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ACE2

anti-sense. sense. a. b. c. 1.0. ACE. O kg/ml). 0.8. 0.6. ACE2. 0.4. Elastance (cmH 2. 0.2. WT. ace2 KO. 0.0. WT. ace2 KO. Supplementary Figure 1. c. a. *. Wet / dry ratio. WT, LPS. WT, saline. ace2 KO, saline. ace2 KO, LPS. 8. 7. 6. 5. 4. b.

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Insulin Pdx4

Insulin Pdx4. A. WT. KO. Figure S1. A. Lactate levels (mice). B. NADPH levels (livers). P < 0.03. P < 0.009. Figure S2. Expression levels of glycolytic genes in KO MEFs. A. Expression levels of TCA cycle genes in KO MEFs. B. Figure S3. Expression levels of Pentose Phosphate

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5 ′ probe

Supplemental Fig.1, Hawkins, et al. 5 ′ probe. 3 ′ probe. Het. KO. A. WT. Het. KO. WT. B. KO. Het. WT. 11126. 6667. 1000. 713. 500. 491. 3229. C. exons 2-3. exons 6-8. exons 12-14. B-actin. brain. liver. brain. liver. brain. liver. brain. liver. WT. KO. WT. KO.

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LacZ

WT. KO. c. b. WT. KO. LacZ. LacZ. hippocampus. 5 m l whole blood. H2A. H2A. LacZ. LacZ. 10 m l whole blood. cortex. H2A. H2A. a. LacZ. 15 m l whole blood. H2A. LacZ. 20 m l whole blood. H2A.

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