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Measuring Dynamic Actuation of the Thrombin Aptamer

Measuring Dynamic Actuation of the Thrombin Aptamer. Miguel Rodriguez. Faculty Mentor: Dr. Pranav Shrotriya Grad Student Mentor: Xiao Ma. Outline. Motivation and objectives Approach, methods Current Status of work/current results Work in progress, planned work and timeline. Motivation.

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Measuring Dynamic Actuation of the Thrombin Aptamer

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  1. Measuring Dynamic Actuation of the Thrombin Aptamer Miguel Rodriguez Faculty Mentor: Dr. Pranav Shrotriya Grad Student Mentor: Xiao Ma

  2. Outline • Motivation and objectives • Approach, methods • Current Status of work/current results • Work in progress, planned work and timeline

  3. Motivation • Integration of Biological and Mechanical Systems • Size and Shape • Communication Wong, Almquist, and Melosh (2010)

  4. Motivation • Thrombin Protein • Fibrinogen Fibrin • Thrombosis (too much fibrin) • Hemorrhage (not enough fibrin) • Anticoagulants • Heparin • DNA Aptamer • Objective: • Specific vs. Non-Specific Binding • Affect binding with electric field

  5. Experimental Design • Test Binding • Poly A + Thrombin Protein • Thrombin Aptamer + Gamma-Thrombin • Thrombin Aptamer + Thrombin Protein • Apply Electric Field

  6. Experimental Design • Produce Pattern

  7. Experimental Design • Apply Electric Field

  8. Methods • Micro-contact Printing • Poly(dimethylsiloxane) (PDMS) • Stiff but flexible • hydrophobic

  9. Methods • Atomic Force Microscope (AFM) • Measure topography of patterned surfaces

  10. Current Results

  11. Current Results

  12. Planned Work • Further Analyze Data • Test Gamma Thrombin-Aptamer Binding • Apply Electric Field to Thrombin-Aptamer Molecules

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